Nanjing University of Chinese Medicine School of Pharmacy, 138 Xianlin Avenue, Nanjing, 210023, China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
Arch Microbiol. 2022 Jul 2;204(7):448. doi: 10.1007/s00203-022-03084-5.
Rhizoma Alismatis, a commonly used traditional Chinese medicine, is the dried tuber of Alisma orientale and Alisma A. plantago-aquatica, mainly cultivated in Fujian and Sichuan provinces (China), respectively. Studies have shown that the rhizosphere microbiome is a key factor determining quality of Chinese medicinal plants. Here we applied metagenomics to investigate the rhizosphere microbiome of Alisma in Fujian and Sichuan, focusing on its structure and function and those genes involved in protostane triterpenes biosynthesis. The dominant phyla were Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, and Gemmatimonadetes. Compared with Fujian, the rhizosphere of Sichuan has a greater α diversity and stronger microbial interactions but significantly lower relative abundance of archaea. Microbes with disease-suppressing functions were more abundant in Sichuan than Fujian, but vice versa for those with IAA-producing functions. Gemmatimonas, Anaeromyxobacter, and Pseudolabrys were the main contributors to the potential functional difference in two regions. Genes related to protostane triterpenes biosynthesis were enriched in Fujian. Steroidobacter, Pseudolabrys, Nevskia, and Nitrospira may contribute to the accumulation of protostane triterpenes in Alisma. This work fills a knowledge gap of Alisma's rhizosphere microbiome, providing a valuable reference for studying its beneficial microorganisms.
泽泻，一种常用的传统中药，是泽泻属和慈姑属植物的干燥根茎，主要在中国的福建和四川种植。研究表明，根际微生物组是决定中药材质量的关键因素。本研究应用宏基因组学方法研究了福建和四川泽泻的根际微生物组，重点关注其结构和功能，以及参与原甾烷三萜生物合成的基因。优势菌群为变形菌门、放线菌门、绿弯菌门、酸杆菌门和芽单胞菌门。与福建相比，四川的根际具有更高的 α 多样性和更强的微生物相互作用，但古菌的相对丰度显著较低。具有抑菌功能的微生物在四川比福建更丰富，而具有 IAA 产生功能的微生物则相反。Gemmatimonas、Anaeromyxobacter 和 Pseudolabrys 是两个地区潜在功能差异的主要贡献者。与原甾烷三萜生物合成相关的基因在福建富集。Steroidobacter、Pseudolabrys、Nevskia 和 Nitrospira 可能有助于泽泻中原甾烷三萜的积累。本研究填补了泽泻根际微生物组的知识空白，为研究其有益微生物提供了有价值的参考。
