School of Life Sciences, University of Nottingham, Life Sciences Building, University Park, Nottingham, NG7 2RD, UK.
Arch Toxicol. 2020 Jun;94(6):2097-2112. doi: 10.1007/s00204-020-02757-8. Epub 2020 May 5.
Dendritic cells (DCs) are professional antigen presenting cells that play a critical role in bridging innate and adaptive immunity. Numerous studies have shown that tobacco constituents present in conventional cigarettes affect the phenotype and function of DCs; however, no studies have examined the effects of vapour from E-cigarettes on human DCs. Here, the effects of E-cigarette vapour extract (ECVE) on the phenotype and function of DCs were investigated by creating an in vitro cell culture model using human monocyte-derived DCs (MoDCs). Immature DCs were generated from peripheral blood monocytes and mature DCs were then produced by treatment with LPS or Poly I:C for 24 h. For LPS-matured DCs, 3% ECVE treatment slightly suppressed HLA-DR and CD86 expression, whereas 1% ECVE treatment enhanced IL-6 production. The overall expression of 29 signalling molecules and other cytoplasmic proteins (mainly associated with DC activation) was significantly upregulated in immature DCs by 1% ECVE, and in LPS-treated DCs by 3% ECVE. In particular, the condition that induced IL-6 production also upregulated MAPK pathway activation. These findings indicate that E-cigarette vapour moderately affects human DCs, but the effects are less pronounced than those reported for tobacco smoke.
树突状细胞（DCs）是专业的抗原呈递细胞，在连接先天免疫和适应性免疫方面发挥着关键作用。大量研究表明，传统香烟中的烟草成分会影响 DCs 的表型和功能；然而，目前尚无研究探讨电子烟蒸气对人源 DCs 的影响。在此，我们通过建立人单核细胞来源的树突状细胞（MoDCs）体外细胞培养模型，研究了电子烟蒸气提取物（ECVE）对 DCs 表型和功能的影响。从外周血单核细胞中生成未成熟的 DCs，然后用 LPS 或 Poly I:C 处理 24 小时生成成熟的 DCs。对于 LPS 成熟的 DCs，3%的 ECVE 处理略微抑制 HLA-DR 和 CD86 的表达，而 1%的 ECVE 处理增强了 IL-6 的产生。1%的 ECVE 处理显著上调了不成熟 DCs 中 29 种信号分子和其他胞质蛋白（主要与 DC 激活相关）的总体表达，而 3%的 ECVE 处理则上调了 LPS 处理的 DCs 中的表达。特别是，诱导 IL-6 产生的条件也上调了 MAPK 通路的激活。这些发现表明，电子烟蒸气对人源 DCs 有一定影响，但影响程度不及烟草烟雾报道的那么明显。
