Wickham Jenny, Corna Andrea, Schwarz Niklas, Uysal Betül, Layer Nikolas, Honegger Jürgen B, Wuttke Thomas V, Koch Henner, Zeck Günther
Neurophysics, Natural and Medical Sciences Institute, University of Tübingen, Reutlingen, Germany.
Graduate School of Neural Information Processing/International Max Planck Research School, Tübingen, Germany.
Front Neurosci. 2020 Apr 21;14:283. doi: 10.3389/fnins.2020.00283. eCollection 2020.
Human cerebrospinal fluid (hCSF) has proven advantageous over conventional medium for culturing both rodent and human brain tissue. In addition, increased activity and synchrony, closer to the dynamic states exclusively recorded , were reported in rodent slices and cell cultures switching from artificial cerebrospinal fluid (aCSF) to hCSF. This indicates that hCSF possesses properties that are not matched by the aCSF, which is generally used for most electrophysiological recordings. To evaluate the possible significance of using hCSF as an electrophysiological recording medium, also for human brain tissue, we compared the network and single-cell firing properties of human brain slice cultures during perfusion with hCSF and aCSF. For measuring the overall activity from a majority of neurons within neocortical and hippocampal human slices, we used a microelectrode array (MEA) recording technique with 252 electrodes covering an area of 3.2 × 3.2 mm. A second CMOS-based MEA with 4225 sensors on a 2 × 2 mm area was used for detailed mapping of action potential waveforms and cell identification. We found that hCSF increased the number of active electrodes and neurons and the firing rate of the neurons in the slices and induced an increase in the numbers of single channel and population bursts. Interestingly, not only an increase in the overall activity in the slices was observed, but a reconfiguration of the network could also be detected with specific activation and inactivation of subpopulations of neuronal ensembles. In conclusion, hCSF is an important component to consider for future human brain slice studies, especially for experiments designed to mimic parts of physiology and disease observed .
事实证明，人类脑脊液（hCSF）在培养啮齿动物和人类脑组织方面比传统培养基更具优势。此外，据报道，在从人工脑脊液（aCSF）转换为hCSF的啮齿动物切片和细胞培养物中，活性和同步性增加，更接近专门记录的动态状态。这表明hCSF具有aCSF所不具备的特性，而aCSF通常用于大多数电生理记录。为了评估将hCSF用作电生理记录介质（也用于人类脑组织）的潜在意义，我们比较了在灌注hCSF和aCSF期间人类脑切片培养物的网络和单细胞放电特性。为了测量新皮质和海马体人类切片中大多数神经元的整体活动，我们使用了微电极阵列（MEA）记录技术，该技术有252个电极，覆盖面积为3.2×3.2毫米。另一个基于互补金属氧化物半导体（CMOS）的MEA，在2×2毫米的区域上有4225个传感器，用于详细绘制动作电位波形和细胞识别。我们发现，hCSF增加了切片中活动电极和神经元的数量以及神经元的放电率，并导致单通道爆发和群体爆发的数量增加。有趣的是，不仅观察到切片中整体活动增加，而且还可以检测到网络的重新配置，神经元集合亚群有特定的激活和失活。总之，hCSF是未来人类脑切片研究中需要考虑的一个重要因素，特别是对于旨在模拟观察到的部分生理和疾病的实验。
