Zhao Ning, Li Dan, Guo Bing-Jing, Tao Xin, Lin Xi, Yan Shu-Zhen, Chen Shuang-Lin
College of Life Sciences, Nanjing Normal University, Nanjing, China.
Front Microbiol. 2020 Apr 9;11:643. doi: 10.3389/fmicb.2020.00643. eCollection 2020.
has long been used as a traditional Chinese medicine and its major medicinal active metabolite is hypocrellin, which exhibits outstanding antiviral and antitumor properties. Here we report the 32 Mb draft genome sequence of S4201, encoding 11,332 predicted genes. The genome of is enriched in carbohydrate-active enzymes (CAZy) and pathogenesis-related genes. The phylogenetic tree of S4201 and nine other sequenced species was constructed and its taxonomic status was supported (, ). The genome contains a rich set of secondary metabolite biosynthetic gene clusters, suggesting that strain S4201 has a remarkable capacity to produce secondary metabolites. Overexpression of the zinc finger transcription factor , which is involved in hypocrellin A (HA) biosynthesis, increases HA production when compared with wild type. In addition, a new putative HA biosynthetic pathway is proposed. These results provide a framework to study the mechanisms of infection in bamboo and to understand the phylogenetic relationships of S4201. At the same time, knowledge of the genome sequence may potentially solve the puzzle of HA biosynthesis and lead to the discovery of novel genes and secondary metabolites of importance in medicine and agriculture.
长期以来一直被用作中药，其主要药用活性代谢产物是竹红菌素，具有出色的抗病毒和抗肿瘤特性。在此我们报告了S4201的32 Mb基因组草图序列，预测编码11332个基因。该基因组富含碳水化合物活性酶（CAZy）和病程相关基因。构建了S4201与其他九个已测序物种的系统发育树，并支持了其分类地位（，）。该基因组包含丰富的次生代谢物生物合成基因簇，表明菌株S4201具有产生次生代谢物的显著能力。与野生型相比，参与竹红菌素A（HA）生物合成的锌指转录因子的过表达增加了HA的产量。此外，还提出了一条新的假定HA生物合成途径。这些结果为研究竹子中的感染机制以及了解S4201的系统发育关系提供了一个框架。同时，基因组序列知识可能潜在地解决HA生物合成的难题，并导致发现医学和农业中重要的新基因和次生代谢物。
