Shen Xiao-Ye, Cao Xue-Ting, Huang Xiao-Bo, Zhuo Lan, Yang Hui-Meng, Fan Li, Hou Cheng-Lin
College of Life Science, Capital Normal University, Beijing, Xisanhuanbeilu 105, Haidian, Beijing100048, China Capital Normal University Beijing China.
IMA Fungus. 2025 Feb 20;16:e138572. doi: 10.3897/imafungus.16.138572. eCollection 2025.
-related species are well-known bambusicolous fungi in class, with high value in traditional medicine for producing hypocrellin, as an anticipated photosensitiser. The complete mitogenomes of hypocrellin-producing strains were analysed in the present study, with functional gene variations through comparative genomics and transcriptomics. Five strains (ZZZ816, CNUCC1353PR, JAP103846, CNUCC C72, CNUCC C151) were sequenced, which indicated similar genome characteristics. Two of them possess an extra gene, and the associated variable fragment "" correlates closely with hypocrellin production capacity. Therefore, these five strains were divided into three groups: ZZZ816 and CNUCC1353PR possessing high production efficiency, CNUCC C72 and JAP103846 with low yield and CNUCC C151 as a transition type. The gene expression changes were screened under various conditions. ZZZ816-related species showed significant changes in mitochondrial genes, especially , and , linked closely to hypocrellin synthesis and stress response; expression also consistently correlated with hypocrellin production. JAP103846 group showed a stable expression pattern divergently, except for suppression by blue light. These findings would provide new insights into secondary metabolite regulation and ROS resistance. Above all, this study conducted the comprehensive analysis of -like fungi mitogenomes and functional gene expression, which can update the understanding of fungal evolution and potential for improved hypocrellin production.
相关物种是该类中著名的竹生真菌，在传统医学中因能产生竹红菌素（一种预期的光敏剂）而具有很高价值。本研究分析了产竹红菌素菌株的完整线粒体基因组，并通过比较基因组学和转录组学研究了功能基因变异。对五个菌株（ZZZ816、CNUCC1353PR、JAP103846、CNUCC C72、CNUCC C151）进行了测序，结果表明它们具有相似的基因组特征。其中两个菌株拥有一个额外基因，与之相关的可变片段“”与竹红菌素的生产能力密切相关。因此，这五个菌株被分为三组：ZZZ816和CNUCC1353PR生产效率高，CNUCC C72和JAP103846产量低，CNUCC C151为过渡类型。在不同条件下筛选了基因表达变化。与ZZZ816相关的物种线粒体基因有显著变化，尤其是与竹红菌素合成和应激反应密切相关的基因、和；的表达也始终与竹红菌素的产生相关。JAP103846组表现出不同的稳定表达模式，除了蓝光对其有抑制作用。这些发现将为次生代谢物调控和活性氧抗性提供新的见解。最重要的是，本研究对类真菌线粒体基因组和功能基因表达进行了全面分析，可更新对真菌进化和提高竹红菌素产量潜力的认识。
