Raybould Alan, Burns Andrea
Science, Technology and Innovation Studies, Old Surgeons' Hall, The University of Edinburgh, Edinburgh, United Kingdom.
Global Academy of Agriculture and Food Security, The University of Edinburgh, Midlothian, United Kingdom.
Front Plant Sci. 2020 Apr 16;11:424. doi: 10.3389/fpls.2020.00424. eCollection 2020.
Externally applied dsRNA-based biocontrol products may lead to off-target degradation of messenger RNA in target and non-target organisms. For the purposes of regulatory risk assessment of such products, producing a comprehensive catalog of any off-target effects using profiling methods is unnecessary and would be ineffective in supporting decision-making. Instead, problem formulation should derive criteria that indicate acceptable risk and devise a plan to test the hypothesis that the product meets those criteria. The key to effective risk assessment of dsRNA-based biocontrols is determining whether their properties indicate acceptable or unacceptable risk, not whether they arise from on- or off-target effects of dsRNA.
基于双链RNA的外用生物防治产品可能会导致目标生物和非目标生物中信使核糖核酸的脱靶降解。出于对此类产品进行监管风险评估的目的，使用分析方法编制任何脱靶效应的综合目录既无必要，也无法有效支持决策制定。相反，问题的提出应得出表明可接受风险的标准，并制定一项计划来检验该产品符合这些标准的假设。对基于双链RNA的生物防治产品进行有效风险评估的关键在于确定其特性表明的是可接受还是不可接受的风险，而非其是否源于双链RNA的靶上或脱靶效应。
