Fariello R G, Ghilardi O, Peschechera A, Ramacci M T, Angelucci L
Department of Neurological Sciences, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
Neuropharmacology. 1988 Oct;27(10):1077-80. doi: 10.1016/0028-3908(88)90071-8.
C57 black mice of 3 months of age were sacrificed, and their brain regionally dissected according to a protocol that strickly control for the death-freezing interval of each region. HPLC measurements of tocopherols and oxidized and reduced ubiquinones demonstrated significant regional variations. The substantia nigra had the lowest content of Q10 and a skewed ratio in favor of its oxidized form. Forebrain cholinergic nuclei had also more oxydized than reduced Q10 and in addition the lowest content of tocopherols. These findings suggest that nuclei that show neuronal depletion with age are the ones prone to oxidative stress.
处死3月龄的C57黑鼠，并按照严格控制每个区域死亡至冷冻间隔的方案对其大脑进行区域解剖。对生育三烯酚以及氧化型和还原型泛醌进行高效液相色谱测量，结果显示存在显著的区域差异。黑质的辅酶Q10含量最低，且其氧化形式的比例偏高。前脑胆碱能核团中氧化型泛醌也多于还原型泛醌，此外生育三烯酚含量最低。这些发现表明，随年龄增长出现神经元耗竭的核团易于发生氧化应激。
