Dang Jia-Wen, Lei Xiao-Ping, Li Qing-Ping, Dong Wen-Bin
Department of Newborn Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
Iran J Basic Med Sci. 2019 Jul;22(7):797-805. doi: 10.22038/ijbms.2019.35913.8556.
Asiaticoside (AS) displays anti-inflammation, and anti-apoptosis effect, but the role of AS in hyperoxia-induced lung injury (HILI) treatment is undefined. Therefore, the aim of this study was to investigate the effects of AS on HILI on premature rats and alveolar type II (AEC II) cells.
Sprague-Dawley premature rats (n=25/group) were exposed to 80% O with or without AS. Then, we detected 80% O-induced lung injury and survival rate of premature rat. We tested the concentration of malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidant capacity (TAOC), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β) in premature rats' blood. Then, the AEC II cell apoptosis was observed by Hoechst 33258 staining and flow cytometry. Simultaneously, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) signaling pathway was measured by Western blot.
Our results found that AS-treated group rats had significantly higher survival rates than 80% O group at day 14 (0.05). AS protected HILI, decreased the MPO and MDA concentration, and reversed TAOC level (0.05). AS also downregulated the levels of TNF-α, IL-1β, and IL-6 in the premature rat's blood (0.01). Moreover, AS markedly attenuated AEC II cell apoptosis and increased Nrf2 and Heme oxygenase 1 (HO-1) expression in the nucleus (0.05).
AS showed protective effects on premature rats of HILI and . AS can potentially be developed as a novel agent for the treatment of HILI diseases.
积雪草苷（AS）具有抗炎和抗凋亡作用，但AS在高氧诱导的肺损伤（HILI）治疗中的作用尚不明确。因此，本研究旨在探讨AS对早产大鼠HILI及肺泡Ⅱ型上皮细胞（AEC II）的影响。
将Sprague-Dawley早产大鼠（每组25只）暴露于80%氧气环境中，部分给予AS。然后，检测80%氧气诱导的肺损伤及早产大鼠的存活率。检测早产大鼠血液中丙二醛（MDA）、髓过氧化物酶（MPO）、总抗氧化能力（TAOC）、肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）和白细胞介素1β（IL-1β）的浓度。通过Hoechst 33258染色和流式细胞术观察AEC II细胞凋亡情况。同时，采用蛋白质免疫印迹法检测核因子（红系衍生2）样2（Nrf2）信号通路。
我们的结果发现，在第14天，AS治疗组大鼠的存活率显著高于80%氧气组（P<0.05）。AS对HILI具有保护作用，降低了MPO和MDA浓度，并逆转了TAOC水平（P<0.05）。AS还下调了早产大鼠血液中TNF-α、IL-1β和IL-6的水平（P<0.01）。此外，AS显著减轻了AEC II细胞凋亡，并增加了细胞核中Nrf2和血红素加氧酶1（HO-1）的表达（P<0.05）。
AS对早产大鼠HILI具有保护作用。AS有望开发成为治疗HILI疾病的新型药物。
