Arsenopoulos Konstantinos, Minoudi Styliani, Symeonidou Isaia, Triantafyllidis Alexandros, Katsafadou Angeliki I, Lianou Daphne T, Fthenakis George C, Papadopoulos Elias
Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Laboratory of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Pathogens. 2020 May 3;9(5):347. doi: 10.3390/pathogens9050347.
The study investigated the presence of resistance to benzimidazoles in helminths from ruminant species in Greece through the detection of the Phe/Tyr polymorphism in the amino acid at position 200 of the -tubulin protein. In total, 288 adult female helminths collected from the abomasum of various ruminant animals in Greece were tested. Of these, 96 were collected from sheep, 96 from goats, 48 from cattle, and 48 from buffaloes. The frequencies of the homozygous and heterozygous resistant genotypes at the position 200 of the -tubulin gene of helminths recovered from sheep were 96.9% and 3.1%, respectively. The frequencies of the homozygous and heterozygous resistant genotypes, respectively, were 100.0% and 0.0% in helminths from goats, 25.0% and 75.0% in helminths from cattle and 8.3% and 91.7% in helminths from buffaloes. In all parasitic populations, no homozygous susceptible genotypes were detected. The present study highlighted, for the first time, the emergence of benzimidazole-resistant in goats, cattle, and buffaloes in Greece, using an allele-specific PCR. It is postulated that benzimidazole-resistant alleles were transferred from sheep or goats to cattle and buffaloes at the commonly grazing pastures in Greece.
该研究通过检测β-微管蛋白200位氨基酸处的苯丙氨酸/酪氨酸多态性，调查了希腊反刍动物体内蠕虫对苯并咪唑的抗药性情况。总共对从希腊各种反刍动物皱胃中采集的288条成年雌性蠕虫进行了检测。其中，96条来自绵羊，96条来自山羊，48条来自牛，48条来自水牛。从绵羊体内回收的蠕虫β-微管蛋白基因200位的纯合抗性基因型和杂合抗性基因型频率分别为96.9%和3.1%。来自山羊的蠕虫中，纯合抗性基因型和杂合抗性基因型频率分别为100.0%和0.0%；来自牛的蠕虫中分别为25.0%和75.0%；来自水牛的蠕虫中分别为8.3%和91.7%。在所有寄生虫种群中，均未检测到纯合敏感基因型。本研究首次利用等位基因特异性PCR揭示了希腊山羊、牛和水牛体内出现苯并咪唑抗性的情况。据推测，苯并咪唑抗性等位基因是在希腊的公共放牧牧场从绵羊或山羊转移到牛和水牛身上的。
