Center for Advanced Lung Disease and Lung Transplantation, University of South Florida, Tampa, FL, USA.
Division of Pulmonary, Critical Care & Sleep Medicine, University of South Florida/Tampa General Hospital, University of South Florida, Tampa, FL, USA.
Respir Res. 2020 May 6;21(1):104. doi: 10.1186/s12931-020-01367-3.
Recent studies suggest that alterations in lung microbiome are associated with occurrence of chronic lung diseases and transplant rejection. To investigate the host-microbiome interactions, we characterized the airway microbiome and metabolome of the allograft (transplanted lung) and native lung of single lung transplant recipients.
BAL was collected from the allograft and native lungs of SLTs and healthy controls. 16S rRNA microbiome analysis was performed on BAL bacterial pellets and supernatant used for metabolome, cytokines and acetylated proline-glycine-proline (Ac-PGP) measurement by liquid chromatography-high-resolution mass spectrometry.
In our cohort, the allograft airway microbiome was distinct with a significantly higher bacterial burden and relative abundance of genera Acinetobacter & Pseudomonas. Likewise, the expression of the pro-inflammatory cytokine VEGF and the neutrophil chemoattractant matrikine Ac-PGP in the allograft was significantly higher. Airway metabolome distinguished the native lung from the allografts and an increased concentration of sphingosine-like metabolites that negatively correlated with abundance of bacteria from phyla Proteobacteria.
Allograft lungs have a distinct microbiome signature, a higher bacterial biomass and an increased Ac-PGP compared to the native lungs in SLTs compared to the native lungs in SLTs. Airway metabolome distinguishes the allografts from native lungs and is associated with distinct microbial communities, suggesting a functional relationship between the local microbiome and metabolome.
最近的研究表明，肺部微生物组的改变与慢性肺部疾病和移植排斥的发生有关。为了研究宿主-微生物组的相互作用，我们对单肺移植受者的同种异体肺（移植肺）和原生肺的气道微生物组和代谢组进行了特征描述。
从 SLT 和健康对照者的同种异体肺和原生肺收集 BAL。对 BAL 细菌沉淀和上清液进行 16S rRNA 微生物组分析，用于通过液相色谱-高分辨率质谱法测量代谢组、细胞因子和乙酰化脯氨酸-甘氨酸-脯氨酸（Ac-PGP）。
在我们的队列中，同种异体气道微生物组具有明显的特征，细菌负荷和不动杆菌属和假单胞菌属的相对丰度明显更高。同样，同种异体中促炎细胞因子 VEGF 和嗜中性粒细胞趋化因子 matrikine Ac-PGP 的表达也明显更高。气道代谢组将原生肺与同种异体肺区分开来，并且鞘氨醇样代谢物的浓度增加，与来自变形菌门的细菌丰度呈负相关。
与 SLT 中的原生肺相比，同种异体肺具有明显的微生物组特征，细菌生物量更高，Ac-PGP 增加。气道代谢组将同种异体肺与原生肺区分开来，并与独特的微生物群落相关，表明局部微生物组和代谢组之间存在功能关系。
