State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3062-3068. doi: 10.1099/ijsem.0.004130.
A Gram-stain-negative, aerobic, rod-shaped and motile bacterial strain, designated L53-10-65, was isolated from deep seawater of the South China Sea. Strain L53-10-65 was found to grow at 4-41 °C (optimum, 28 °C), at pH 5.0-9.0 (pH 7.0-8.0) and in 0-7 % (w/v) NaCl (2 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain L53-10-65 fell within the genus , with the highest similarity to IO390501 (98.4 %), followed by '' L15 (97.7 %) and IFO 13584 (96.8 %). The digital DNA-DNA hybridization (dDDH) values between strain L53-10-65 and the three relatives above were 43.7, 21.5 and 20.9 %, respectively; the average nucleotide identity (ANI) values were 91.3, 78.4 and 76.8 %, respectively. These values were below the 70 % dDDH and 95-96 % ANI thresholds for bacterial species delineation. The major cellular fatty acids of strain L53-10-65 were summed feature 8 (C 7 and/or C 6), C 7 11-methyl and C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The respiratory quinone was identified as Q-10. The DNA G+C content of strain L53-10-65 was 61.3 mol%. Based on the results of phylogenetic analysis and distinctive phenotypic characteristics, strain L53-10-65 is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the species is L53-10-65 (=MCCC 1A05139=KCTC 72888). Moreover, we propose that is a later heterotypic synonym of based on the present results.
一株革兰氏阴性、需氧、杆状且能运动的细菌菌株，被命名为 L53-10-65，从南海深海水中分离得到。菌株 L53-10-65 被发现可以在 4-41°C（最佳温度为 28°C）、pH 值为 5.0-9.0（最佳 pH 值为 7.0-8.0）和 0-7%（w/v）NaCl（最佳盐浓度为 2%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 L53-10-65 属于属，与 IO390501（98.4%）的相似度最高，其次是 L15（97.7%）和 IFO 13584（96.8%）。菌株 L53-10-65 与上述三种亲缘关系菌株之间的数字 DNA-DNA 杂交（dDDH）值分别为 43.7%、21.5%和 20.9%；平均核苷酸同一性（ANI）值分别为 91.3%、78.4%和 76.8%。这些值均低于细菌种间划分的 70% dDDH 和 95-96%ANI 阈值。菌株 L53-10-65 的主要细胞脂肪酸为 C7 和/或 C6 特征脂肪酸、C7 11-甲基和 C18:1ω7c。主要极性脂为双磷脂酰甘油、磷脂酰甘油和糖脂。呼吸醌为 Q-10。菌株 L53-10-65 的 DNA G+C 含量为 61.3mol%。基于系统发育分析和独特表型特征的结果，菌株 L53-10-65 被鉴定为属的一个新种，建议将其命名为 sp. nov.。该种的模式菌株为 L53-10-65（=MCCC 1A05139=KCTC 72888）。此外，根据目前的结果，我们建议将基于的种名视为一个异名。
