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sp. nov.,分离自白令海表面沉积物。

sp. nov., isolated from surface sediment of the Bering Sea.

机构信息

Polar Research Institute of China, Ministry of Natural Resources, Shanghai 200136, PR China.

College of Marine Science, Shandong University, Weihai, 264209, PR China.

出版信息

Int J Syst Evol Microbiol. 2021 Aug;71(8). doi: 10.1099/ijsem.0.004995.

Abstract

Strain S02 was isolated from a surface sediment sample collected from the Bering Sea (64.3361° N, 170.9541° W). The cells were Gram-stain-negative, motile and rod-shaped. The temperature range for growth was 4-25 °C and the pH for growth was 5.5-9.0, with optimum growth occurring at 20-25 °C and pH 7.0-8.0. Growth occurred in the presence of 0-7 % (w/v) NaCl (optimum, 2-5 %). Strain S02 had menaquinone-8 as the major respiratory quinone and summed feature 8 (C 7 and/or C 6), C16:0, C cyclo, summed feature 3 (C 7 /C 7), C and C as major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two glycolipids. The genomic DNA G+C content was approximately 63.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S02 belonged to the genus . Strain S02 showed the highest sequence similarities to Cr7-05 (97.5 %), CM5-1 (97.7 %), KMM 9415 (97.4 %), E84 (97.44 %), LIV5 (97.1 %) and DSM 17137 (96.7 %). On the basis of phylogenetic analyses and phenotypic characteristics, a novel species of the genus , sp. nov., is proposed, with the type strain S02 (=JCM 33772=CCTCC AB 2019343).

摘要

菌株 S02 是从白令海(北纬 64.3361°,西经 170.9541°)的表层沉积物样本中分离出来的。细胞革兰氏染色阴性,可运动,呈杆状。生长温度范围为 4-25°C,生长 pH 值为 5.5-9.0,最佳生长条件为 20-25°C 和 pH 7.0-8.0。在 0-7%(w/v)NaCl(最佳为 2-5%)存在的情况下可以生长。菌株 S02 的主要呼吸醌为menaquinone-8,特征脂肪酸为 sum feature 8(C 7 和/或 C 6)、C 16:0、C cyclo、sum feature 3(C 7 /C 7)和 C 18:1ω7c 和 C 18:1ω6c。主要极性脂为二磷脂酰甘油、磷脂酰甘油和两种糖脂。基因组 DNA G+C 含量约为 63.8mol%。基于 16S rRNA 基因序列的系统发育分析表明,菌株 S02 属于 属。菌株 S02 与 Cr7-05(97.5%)、CM5-1(97.7%)、KMM 9415(97.4%)、E84(97.44%)、LIV5(97.1%)和 DSM 17137(96.7%)的序列相似性最高。根据系统发育分析和表型特征,提出了一个新的属 种,即 sp. nov.,其模式菌株为 S02(=JCM 33772=CCTCC AB 2019343)。

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