Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People's Republic of China.
Curr Microbiol. 2021 Jul;78(7):2806-2814. doi: 10.1007/s00284-021-02538-1. Epub 2021 May 28.
A novel Gram-stain-negative, non-motile, and rod-shaped bacterial strain, designated as 6D36, was isolated from mangrove soil and characterized by using a polyphasic taxonomic approach. Strain 6D36 was found to grow at 10-37 °C (optimum, 28 °C), at pH 6.0-9.0 (optimum, 7.0) and in 0-8% (w/v) NaCl (optimum, 3%). The predominant cellular fatty acids of strain 6D36 were summed feature 8 (C ω7c and/or C ω6c) and C ω6c; the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and sphingoglycolipid; the sole respiratory quinone was Q-10. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain 6D36 fell into the genus Qipengyuania and was closely related to "Erythrobacter mangrovi" MCCC 1K03690 (98.5%), Qipengyuania citrea CGMCC 1.8703 (97.6%), and Qipengyuania pelagi JCM 17468 (97.4%). The phylogenomic analysis indicated that strain 6D36 formed an independent branch distinct from reference-type strains of species within this genus. The digital DNA-DNA hybridization and average nucleotide identity values between strain 6D36 and the three type strains above were, respectively, 20.2-21.3% and 79.5-81.5%, of which were far below their respective threshold for species definition, implying that the strain represents a novel genospecies. The genomic DNA G + C content was 63.3%. Based on phenotypic and genotypic characteristics, strain 6D36 is concluded to represent a novel species of the genus Qipengyuania, for which the name Qipengyuania soli sp. nov., is proposed. The type strain of the species is 6D36 (= GDMCC 1.1977 = KCTC 82333).
一株新的革兰氏阴性、非运动和杆状细菌菌株，被命名为 6D36，从红树林土壤中分离出来，并通过多相分类学方法进行了表征。菌株 6D36 被发现可以在 10-37°C（最佳温度为 28°C）、pH 值 6.0-9.0（最佳 pH 值为 7.0）和 0-8%（w/v）NaCl（最佳浓度为 3%）下生长。菌株 6D36 的主要细胞脂肪酸为 summed feature 8（C ω7c 和/或 C ω6c）和 C ω6c；主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和神经酰胺糖脂；唯一的呼吸醌为 Q-10。基于 16S rRNA 基因序列的系统发育分析表明，菌株 6D36 属于 Qipengyuania 属，与“Erythrobacter mangrovi”MCCC 1K03690（98.5%）、Qipengyuania citrea CGMCC 1.8703（97.6%）和 Qipengyuania pelagi JCM 17468（97.4%）最为密切相关。基因组分析表明，菌株 6D36 形成了一个与该属内参考种菌株不同的独立分支。菌株 6D36 与上述三种模式菌株的数字 DNA-DNA 杂交值和平均核苷酸同一性值分别为 20.2-21.3%和 79.5-81.5%，均远低于各自种定义的界限，表明该菌株代表了一个新的种。基因组 DNA G+C 含量为 63.3%。基于表型和基因型特征，菌株 6D36 被认为是 Qipengyuania 属的一个新种，因此提议将其命名为 Qipengyuania soli sp. nov.。该种的模式菌株为 6D36（=GDMCC 1.1977=KCTC 82333）。
