The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3264-3272. doi: 10.1099/ijsem.0.004164.
Four strains (SYSU SYW-1, SYW-2, SYW-3 and XLW-1) were isolated from seawater near the shore in Guangdong Province, China. Cells were Gram-stain-negative, aerobic, non-motile and non-spore-forming. Growth was observed at a temperature range of 16-40 °C (optimum, 32 °C), a pH range of 4-8 (optimum, pH 7) and in the presence of up to 10 % (w/v) NaCl. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and an unidentified phospholipid. The respiratory quinone was ubiquinone 8 (UQ-8), and the predominant fatty acids were C 3-OH, C, C and Cω9. Comparison of 16S rRNA gene and genome sequences confirmed that these strains represented a novel member of the genus , with less than 98.8 % 16S rRNA gene sequence similarity and less than 95 % genomic average nucleotide identity to recognized species. The phylogenetic tree based on 16S rRNA gene sequences and the protein-concatamer tree based on a concatenation of 28 protein marker sequences both indicated that the strains clustered with '' TX07-7308 and '' E95-16, but formed a distinct lineage group among the other members of the genus . The DNA G+C contents of the four strains were determined to be 32.9, 32.7, 32.9 and 32.9 %, respectively (genome). On the basis of phenotypic and genotypic features, the strains are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU SYW-1 (=CGMCC 1.17031=NBRC 113781).
从中国广东省近岸海水中共分离到 4 株菌株（SYSU SYW-1、SYW-2、SYW-3 和 XLW-1）。细胞革兰氏染色阴性，需氧，不运动，不产芽孢。生长温度范围为 16-40°C（最佳温度 32°C），pH 值范围为 4-8（最佳 pH 值 7），可耐受高达 10%（w/v）的 NaCl。主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和一种未鉴定的磷脂。呼吸醌为泛醌 8（UQ-8），主要脂肪酸为 C3-OH、C16:0、C18:1ω9 和 C18:1ω7。16S rRNA 基因和基因组序列的比较证实，这些菌株代表了一个新属的成员，与公认的种的 16S rRNA 基因序列相似性低于 98.8%，基因组平均核苷酸同一性低于 95%。基于 16S rRNA 基因序列的系统发育树和基于 28 个蛋白标记序列串联的蛋白联体树均表明，这些菌株与“TX07-7308”和“E95-16”聚在一起，但在属内的其他成员中形成了一个独特的谱系群。4 株菌的 DNA G+C 含量分别为 32.9%、32.7%、32.9%和 32.9%（基因组）。基于表型和基因型特征，这些菌株被认为代表了一个新种的属，建议将其命名为 sp. nov.。模式菌株为 SYSU SYW-1（=CGMCC 1.17031=NBRC 113781）。
