Breathnach A S, Pätzold H C, Robins E J, Bhasin Y D, Ethridge L, Nazzaro-Porro M
Department of Anatomy and Cell Biology, St. Mary's Hospital Medical School, London, United Kingdom.
Pigment Cell Res. 1988;1(5):315-25. doi: 10.1111/j.1600-0749.1988.tb00126.x.
Basic LM, TEM, SEM, and FFR appearances of a pure line of normal human melanocytes derived from foreskin, and a human melanoma line, in cell culture are described. Normal melanocyte cultures exhibit side by side, cells of widely different melanogenic activities--possible clones--and melanosomes of bizarre shape and internal structure are frequent. Aggregates of melanosomes, with or without associated amorphous material, and with no discernible limiting membrane are present within many cells, and occasional simple specialised contacts occur between apposed cells. On replicas of plasma membrane of normal melanocytes, particle densities and diameters on P and E fracture faces were within the ranges for cells in general, and equivalent data for the melanoma cells were not significantly different. Similarly, there was no difference in density of distribution or diameter of nuclear pores between the normal and the tumoural cells.
描述了来自包皮的正常人黑素细胞纯系以及人黑素瘤细胞系在细胞培养中的基本光镜、透射电镜、扫描电镜和血流储备分数表现。正常黑素细胞培养物中，并排存在具有广泛不同黑素生成活性的细胞——可能是克隆——并且形状怪异和内部结构奇特的黑素小体很常见。许多细胞内存在黑素小体聚集体，有或没有相关的无定形物质，且没有可辨别的界限膜，相邻细胞之间偶尔会出现简单的特化接触。在正常黑素细胞质膜的复制品上，P面和E面断裂面上的颗粒密度和直径在一般细胞的范围内，黑素瘤细胞的等效数据没有显著差异。同样，正常细胞和肿瘤细胞之间核孔的分布密度或直径也没有差异。
