Rhodes A R, Seki Y, Fitzpatrick T B, Stern R S
Department of Dermatology, Massachusetts General Hospital, Boston 02114.
Cancer. 1988 Jan 15;61(2):358-69. doi: 10.1002/1097-0142(19880115)61:2<358::aid-cncr2820610227>3.0.co;2-#.
Abnormal-looking melanosomes are observed commonly in both intraepidermal melanocytes of cutaneous melanoma and dysplastic melanocytic nevi (DMN). This was investigated by using transmission electron microscopic examination to determine the percentage of abnormal melanosomes among 8267 melanosomes assessed in at least five solitary intraepidermal basal unit melanocytes from each of five specimens of DMN, superficial spreading melanoma (SSM), common acquired nevomelanocytic nevi (NMN), and normal skin (NS) adjacent to DMN. The percentage of abnormal melanosomes in DMN (mean + SD, 44 + 23%) was seven times greater than that in NMN (6 + 7%) and 22 times greater than that in NS (2 + 5% [P less than 0.001, both comparisons]), but only 80% that in SSM (57 + 19% [P less than 0.02]). Melanocyte area, nuclear area, and the ratio of nuclear area to cytoplasmic area did not account for the observed differences. Melanosomal alterations may be a useful marker of atypicality in melanocytic tumors.
在皮肤黑色素瘤的表皮内黑色素细胞和发育异常的黑素细胞痣（DMN）中，通常都能观察到外观异常的黑素小体。通过透射电子显微镜检查进行了此项研究，以确定在来自五个DMN标本、浅表扩散性黑色素瘤（SSM）、常见获得性痣黑素细胞痣（NMN）以及与DMN相邻的正常皮肤（NS）的至少五个孤立表皮基底单位黑色素细胞中评估的8267个黑素小体中异常黑素小体的百分比。DMN中异常黑素小体的百分比（均值±标准差，44±23%）比NMN（6±7%）高7倍，比NS（2±5%[P<0.001，两项比较均如此]）高22倍，但仅为SSM中（57±19%[P<0.02]）的80%。黑色素细胞面积、核面积以及核面积与细胞质面积的比值并不能解释所观察到的差异。黑素小体改变可能是黑素细胞肿瘤非典型性的一个有用标志物。
