单脉冲电磁场恢复失神经/废用性骨质疏松症小鼠的骨量和微结构。

Single pulsed electromagnetic field restores bone mass and microarchitecture in denervation/disuse osteopenic mice.

机构信息

Medical Device Innovation Center, National Cheng Kung University, Tainan, Taiwan; Department of Orthopedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Bone and Joint Research Center, National Cheng Kung University, Tainan, Taiwan.

Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan.

出版信息

Med Eng Phys. 2020 Jun;80:52-59. doi: 10.1016/j.medengphy.2019.10.004. Epub 2020 May 4.

DOI:10.1016/j.medengphy.2019.10.004

PMID:32376078

Abstract

Pulsed electromagnetic fields (PEMFs) have been proposed to treat bone loss. However, time-consuming is the main complaint. A time-saving and effective treatment is of expectation. Previously, we showed a 3 min daily of single pulsed electromagnetic field (SPEMF) accelerated bone formation of long bone defect in mice. Here we compared the effect of SPEMF with PEMF for treating denervation/disuse osteopenic mice. Healthy mice were divided into 3 groups: intact mice (INT), INT + PEMF, and INT + SPEMF. Induced osteopenic mice were divided to osteopenia (IOP), IOP + PEMF, and IOP + SPEMF groups. The PEMF treated groups were subjected to daily 8 h PEMF(15 Hz, 18 G) exposure, while SPEMF treated groups were daily 3 min SPEMF(0.2 Hz, 1 T) exposure. BMD was evaluated every two weeks during the 12 weeks of treatment. Microarchitecture was evaluated on week 12. SPEMF significantly reversed bone loss in IOP mice as early as 6 weeks post-treatment, while PEMF reversed bone loss after 8 weeks. Bone volume was significantly increased in the IOP + PEMF and IOP + SPEMF group. Besides, bone volume and trabecular number of IOP + SPEMF mice were restored to the levels of INT mice in 12 weeks. Our finding suggests SPEMF increased BMD and restored microarchitecture of disuse osteopenic mice to healthy level.

摘要

脉冲电磁场（PEMFs）已被提议用于治疗骨质疏松症。然而，耗时长是主要的抱怨。人们期待一种省时有效的治疗方法。之前，我们展示了每天 3 分钟的单次脉冲电磁场（SPEMF）加速了小鼠长骨缺损的骨形成。在这里，我们比较了 SPEMF 与 PEMF 治疗去神经/废用性骨质疏松症小鼠的效果。健康小鼠分为 3 组：完整组（INT）、INT+PEMF 组和 INT+SPEMF 组。诱导骨质疏松症小鼠分为骨质疏松症组（IOP）、IOP+PEMF 组和 IOP+SPEMF 组。PEMF 治疗组每天接受 8 小时 PEMF（15Hz，18G）照射，而 SPEMF 治疗组每天接受 3 分钟 SPEMF（0.2Hz，1T）照射。在 12 周的治疗期间，每两周评估一次 BMD。第 12 周评估微结构。SPEMF 早在治疗后 6 周就显著逆转了 IOP 小鼠的骨丢失，而 PEMF 在 8 周后才逆转了骨丢失。IOP+PEMF 和 IOP+SPEMF 组的骨体积显著增加。此外，在 12 周时，IOP+SPEMF 组小鼠的骨体积和小梁数量恢复到 INT 组小鼠的水平。我们的研究结果表明，SPEMF 增加了 BMD，并使废用性骨质疏松症小鼠的微结构恢复到健康水平。