Medical Device Innovation Center, National Cheng Kung University, Tainan, Taiwan; Department of Orthopedics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Bone and Joint Research Center, National Cheng Kung University, Tainan, Taiwan.
Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan.
Med Eng Phys. 2020 Jun;80:52-59. doi: 10.1016/j.medengphy.2019.10.004. Epub 2020 May 4.
Pulsed electromagnetic fields (PEMFs) have been proposed to treat bone loss. However, time-consuming is the main complaint. A time-saving and effective treatment is of expectation. Previously, we showed a 3 min daily of single pulsed electromagnetic field (SPEMF) accelerated bone formation of long bone defect in mice. Here we compared the effect of SPEMF with PEMF for treating denervation/disuse osteopenic mice. Healthy mice were divided into 3 groups: intact mice (INT), INT + PEMF, and INT + SPEMF. Induced osteopenic mice were divided to osteopenia (IOP), IOP + PEMF, and IOP + SPEMF groups. The PEMF treated groups were subjected to daily 8 h PEMF(15 Hz, 18 G) exposure, while SPEMF treated groups were daily 3 min SPEMF(0.2 Hz, 1 T) exposure. BMD was evaluated every two weeks during the 12 weeks of treatment. Microarchitecture was evaluated on week 12. SPEMF significantly reversed bone loss in IOP mice as early as 6 weeks post-treatment, while PEMF reversed bone loss after 8 weeks. Bone volume was significantly increased in the IOP + PEMF and IOP + SPEMF group. Besides, bone volume and trabecular number of IOP + SPEMF mice were restored to the levels of INT mice in 12 weeks. Our finding suggests SPEMF increased BMD and restored microarchitecture of disuse osteopenic mice to healthy level.
脉冲电磁场（PEMFs）已被提议用于治疗骨质疏松症。然而，耗时长是主要的抱怨。人们期待一种省时有效的治疗方法。之前，我们展示了每天 3 分钟的单次脉冲电磁场（SPEMF）加速了小鼠长骨缺损的骨形成。在这里，我们比较了 SPEMF 与 PEMF 治疗去神经/废用性骨质疏松症小鼠的效果。健康小鼠分为 3 组：完整组（INT）、INT+PEMF 组和 INT+SPEMF 组。诱导骨质疏松症小鼠分为骨质疏松症组（IOP）、IOP+PEMF 组和 IOP+SPEMF 组。PEMF 治疗组每天接受 8 小时 PEMF（15Hz，18G）照射，而 SPEMF 治疗组每天接受 3 分钟 SPEMF（0.2Hz，1T）照射。在 12 周的治疗期间，每两周评估一次 BMD。第 12 周评估微结构。SPEMF 早在治疗后 6 周就显著逆转了 IOP 小鼠的骨丢失，而 PEMF 在 8 周后才逆转了骨丢失。IOP+PEMF 和 IOP+SPEMF 组的骨体积显著增加。此外，在 12 周时，IOP+SPEMF 组小鼠的骨体积和小梁数量恢复到 INT 组小鼠的水平。我们的研究结果表明，SPEMF 增加了 BMD，并使废用性骨质疏松症小鼠的微结构恢复到健康水平。
