Vincent Obstetrics and Gynecology Department, Division of Female Pelvic Medicine and Reconstructive Surgery, Massachusetts General Hospital, Boston, MA, United States.
Department of Obstetrics, Gynecology & Reproductive Biology, Harvard Medical School, Boston, MA, United States.
Sci Rep. 2020 May 6;10(1):7625. doi: 10.1038/s41598-020-63652-x.
Half of postmenopausal women experience genitourinary syndrome of menopause, for which many use lubricating vaginal products. The effect of vaginal products on uropathogenic and commensal vaginal bacteria is poorly understood. We evaluated the effect of five common vaginal products (KY Jelly, Replens Silky Smooth lubricant, coconut oil, Replens Long-Lasting moisturizer or Trimo-San) on growth and viability of Escherichia coli and Lactobacillus crispatus. Bacteria were co-cultured products alone and in the presence of both vaginal epithelial cells and selected products. Bacterial growth was compared between conditions using an unpaired t-test or ANOVA, as appropriate. All products except for coconut oil significantly inhibited growth of laboratory and clinical strains of Escherichia coli (p < 0.02). Only two products (Replens Long-Lasting moisturizer and Trimo-San) significantly inhibited growth of Lactobacillus crispatus (p < 0.01), while the product Replens Silky Smooth stimulated growth (p < 0.01). Co-culture of selected products in the presence of vaginal epithelial cells eliminated the inhibitory effects of the products on E. coli. In conclusion, in vitro exposure to vaginal moisturizing and lubricating products inhibited growth of Escherichia coli, though the inhibition was mitigated by the presence of vaginal epithelial cells. Lactobacillus crispatus demonstrated less growth inhibition than Escherichia coli.
一半的绝经后妇女患有泌尿生殖系统绝经综合征，许多人为此使用阴道润滑产品。这些产品对尿路病原体和共生阴道细菌的影响知之甚少。我们评估了五种常见阴道产品（KY 凝胶、Replens 丝滑润滑剂、椰子油、Replens 长效保湿剂或 Trimo-San）对大肠杆菌和乳酸杆菌生长和活力的影响。将细菌单独与阴道上皮细胞和选定的产品一起进行共培养，并在不同条件下比较细菌的生长情况，使用非配对 t 检验或 ANOVA，视情况而定。除了椰子油外，所有产品都显著抑制了实验室和临床大肠杆菌菌株的生长（p<0.02）。只有两种产品（Replens 长效保湿剂和 Trimo-San）显著抑制了乳酸杆菌的生长（p<0.01），而 Replens 丝滑润滑剂刺激了其生长（p<0.01）。在存在阴道上皮细胞的情况下，共培养选定的产品消除了产品对大肠杆菌生长的抑制作用。总之，体外接触阴道保湿和润滑产品会抑制大肠杆菌的生长，但阴道上皮细胞的存在减轻了这种抑制作用。与大肠杆菌相比，乳酸杆菌的生长抑制作用较小。
