The experiments were conducted for the estimation of the mitigating effect of the static magnetic field (SMF of 200 mT for 1 h) treatment on soybean under ambient UV-B stress. The SMF treated (MT) and untreated (UT) seeds were grown inside iron cages covered with polyester filters for the purpose to filter UV-A + B (< 400 nm) and UV-B (< 300 nm) radiations, polythene filter control (FC) transparent for UV (280-400 nm), and open controls (OC) were without any filters. Our results indicated that specific leaf weight, efficiency of PS II, activity of carbonic anhydrase (CA) and nitrogenase (NRA), nucleic acid and protein content, nitric oxide (NO) and yield were significantly decreased in plants of untreated seeds under UV-B stress. SMF treatment to the soybean seeds was observed to mitigate the adverse effect of ambient UV-B with a significant enhancement in above-measured parameters in plants when compared with plants of untreated seeds grown under OC/FC conditions. Chlorophyll fluorescence transition curve (OJIP-curve) from SMF treated and UV excluded plants has shown a higher fluorescence yield especially for I-P phase as compared to the plants grown in ambient UV-B stress. Reduction in the level of superoxide anion radicle ( ), hydrogen peroxide (HO), malondialdehyde (MDA) and proline content with a remarkable increase in DNA, RNA, protein and NO content, increased photosynthetic efficiency and nitrogen fixation in the leaves of soybean suggested the ameliorating effect of SMF pre-treatment against ambient UV-B induced damage. Consequently, SMF-pretreatment increased the tolerance of soybean seedlings to ambient UV-B stress as compared to the untreated seeds. The increase in carbon and nitrogen fixation ability due to SMF pre-treatment and the omission of solar UV radiation impact can be a direction for the purpose to improve the crop yield. Evaluation of the consequences of SMF treated seeds under ambient UV-B stress, and the plants from untreated seeds under solar UV exclusion indicated parallelism among the two effects.