Singh Harish Chandra, Tiwari Vandana, Meena Baleshwar, Tiwari Avinash, Rana Tikam Singh
Molecular Systematics Laboratory, Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, 226001, India.
School of Studies in Botany, Jiwaji University, Gwalior, Madhya Pradesh, 474011, India.
Biochem Genet. 2024 Sep 2. doi: 10.1007/s10528-024-10908-0.
Bergenia ciliata (Haw.) Sternb. is a perennial medicinal herb distributed in Indian Himalayan Region (IHR). A total of eight populations of B. ciliata were collected from diverse locales of IHR, and 17 EST-SSR markers were used in this study. The present study revealed moderate genetic diversity at the locus level with the mean number of alleles (Na = 7.823), mean number effective of alleles (Ne = 3.375), mean expected heterozygosity (He = 0.570), and mean Shannon's diversity index (I = 1.264). The MSR (He = 0.543, I = 1.067) and DRJ populations (He = 0.309, I = 0.519) revealed the highest and lowest genetic diversity at the population level, respectively. AMOVA analysis showed that 81.76% of genetic variation was within populations, 10.55% was among populations, and 7.69% was among the regions. In addition, a moderate to high level of differentiation was found among the populations (F = 0.182), which could be indicative of low to moderate gene flow (Nm = 0.669) in the B. ciliata populations. UPGMA and PCoA analysis revealed that eight populations could be differentiated into two groups, while the structure analysis of the 96 individuals differentiated into three groups. The Mantel test showed a positive relationship between genetic and geographical distance. The findings of this study will provide the development of conservation and germplasm management strategies for this valuable medicinal species.
岩白菜（Bergenia ciliata (Haw.) Sternb.）是一种分布于印度喜马拉雅地区（IHR）的多年生药用草本植物。本研究从IHR的不同地点收集了总共8个岩白菜种群，并使用了17个EST-SSR标记。本研究揭示了该位点水平上的中等遗传多样性，平均等位基因数（Na = 7.823）、平均有效等位基因数（Ne = 3.375）、平均期望杂合度（He = 0.570）和平均香农多样性指数（I = 1.264）。MSR种群（He = 0.543，I = 1.067）和DRJ种群（He = 0.309，I = 0.519）分别在种群水平上显示出最高和最低的遗传多样性。AMOVA分析表明，81.76%的遗传变异存在于种群内，10.55%存在于种群间，7.69%存在于区域间。此外，在种群间发现了中等至高程度的分化（F = 0.182），这可能表明岩白菜种群中的基因流较低至中等（Nm = 0.669）。UPGMA和PCoA分析表明，8个种群可分为两组，而对96个个体的结构分析则分为三组。Mantel检验显示遗传距离和地理距离之间存在正相关关系。本研究结果将为这种珍贵药用植物的保护和种质管理策略的制定提供依据。
