Ren Wenjing, Li Zhiyuan, Han Fengqing, Zhang Bin, Li Xing, Fang Zhiyuan, Yang Limei, Zhuang Mu, Lv Honghao, Liu Yumei, Wang Yong, Yu Hailong, Zhang Yangyong
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081 China.
Hortic Res. 2020 May 1;7:61. doi: 10.1038/s41438-020-0282-8. eCollection 2020.
Clubroot disease, a major plant root disease caused by , has become one of the most destructive diseases among cultivated cruciferous vegetables. However, clubroot-resistant materials are rare. A few clubroot-resistant cabbage varieties are available on the market, but all are Ogura cytoplasmic male sterile (CMS) types. Therefore, in this study, to reutilize the clubroot-resistant Ogura CMS germplasm of cabbage, a new fertility-restored Ogura CMS material, 16Q2-11, was used as a bridge to transfer the clubroot resistance (CR) gene from the Ogura CMS cytoplasm to the normal cytoplasm by a two-step method (a fertility restoration and cytoplasm replacement method). In the first cross for fertility restoration of Ogura CMS clubroot-resistant cabbage (FRCRC), 16Q2-11 was used as a restorer to cross with Ogura CMS materials containing the CR gene . Eleven -positive progenies were generated, of which four contained : F8-514, F8-620, F8-732 and F8-839. After inoculation with race 4 of , these four -positive individuals showed resistance. Furthermore, F8-514 and F8-839 were then used as male parents in the second cross of FRCRC to cross with cabbage inbred lines, resulting in the successful introgression of the gene into the inbred lines. All offspring produced from this step of cross, which had a normal cytoplasm, showed a high resistance to race 4 of and could be utilized for the breeding of clubroot-resistant cabbage varieties in the future. This is the first time that the Ogura CMS restorer has been used to restore the fertility of Ogura CMS clubroot-resistant cabbages, which could improve germplasm diversity in cabbage and provide a reference method for using CMS germplasm in crops.
根肿病是由[病原体名称未给出]引起的一种主要的植物根部病害，已成为栽培十字花科蔬菜中最具破坏性的病害之一。然而，抗根肿病的材料很少。市场上有一些抗根肿病的甘蓝品种，但都是ogu ra细胞质雄性不育（CMS）类型。因此，在本研究中，为了重新利用甘蓝的抗根肿病ogu ra CMS种质资源，一种新的育性恢复ogu ra CMS材料16Q2 - 11被用作桥梁，通过两步法（育性恢复和细胞质置换法）将ogu ra CMS细胞质中的抗根肿病（CR）基因转移到正常细胞质中。在ogu ra CMS抗根肿病甘蓝育性恢复的第一次杂交（FRCRC）中，16Q2 - 11被用作恢复系与含有CR基因的ogu ra CMS材料杂交。产生了11个阳性后代，其中4个含有[相关基因未明确给出]：F8 - 514、F8 - 620、F8 - 732和F8 - 839。用[病原体名称未给出]的4号生理小种接种后，这4个阳性个体表现出抗性。此外，F8 - 514和F8 - 839随后在FRCRC的第二次杂交中用作父本与甘蓝自交系杂交，从而成功地将[相关基因未明确给出]基因导入自交系。这一步杂交产生的所有具有正常细胞质的后代对[病原体名称未给出]的4号生理小种都表现出高抗性，并且将来可用于抗根肿病甘蓝品种的育种。这是首次使用ogu ra CMS恢复系来恢复ogu ra CMS抗根肿病甘蓝的育性，这可以提高甘蓝的种质多样性，并为在[相关作物未明确给出]作物中利用CMS种质提供一种参考方法。
