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萝卜细胞质雄性不育恢复系R2163对根肿病抗性的改良

Improvement of Resistance to Clubroot Disease in the Ogura CMS Restorer Line R2163 of .

作者信息

Chen Jiao, Li Jiahui, Ma Mengya, Li Bao, Zhou Yuanwei, Pan Yongzhong, Fan Youjun, Yi Bin, Tu Jinxing

机构信息

National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, National Center of Rapeseed Improvement, Huazhong Agricultural University, Wuhan 430070, China.

Yichang Academy of Agricultural Science, Yichang 443009, China.

出版信息

Plants (Basel). 2022 Sep 16;11(18):2413. doi: 10.3390/plants11182413.

Abstract

Oilseed rape () has significant heterosis and Ogura CMS is a major way to use it. Ogura CMS has the advantages of complete and stable male sterility and easy-to-breed maintainers. Therefore, to breed better restorers has become an important goal for this system. Incidentally, clubroot is a soil-borne disease that is difficult to control by fungicidal chemicals, and it has been the main disease of oilseed rape in recent years in China, severely restricting the development of the oilseed rape industry. At present, the most effective method for controlling clubroot disease is to cultivate resistant varieties. One Ogura CMS restorer line (R2163) has shown much better combining ability, but lacks the clubroot disease resistance. This study was carried out to improve R2163 through marker-assisted backcross breeding (MABB). The resistant locus was introduced into the restorer R2163, and we then selected R2163R with clubroot disease resistance. Using the new restorer R2163R as the male parent and the sterile lines 116A and Z11A as the female parent, the improved, new resistant hybrids Kenyouza 741R and Huayouza 706R performed well, providing strong resistance and good agronomic traits. This work advances the utilization of heterosis and breeding for clubroot disease resistance in .

摘要

油菜具有显著的杂种优势,而小仓细胞质雄性不育系(Ogura CMS)是利用杂种优势的主要途径之一。Ogura CMS具有雄性不育彻底、稳定且易于选育保持系的优点。因此,培育优良的恢复系成为该体系的重要目标。顺便提一下,根肿病是一种土传病害,难以用化学杀菌剂防治,近年来已成为我国油菜的主要病害,严重制约了油菜产业的发展。目前,防治根肿病最有效的方法是培育抗病品种。一个小仓细胞质雄性不育恢复系(R2163)表现出较好的配合力,但缺乏根肿病抗性。本研究通过分子标记辅助回交育种(MABB)对R2163进行改良。将抗病位点导入恢复系R2163,选育出具有根肿病抗性的R2163R。以新恢复系R2163R为父本,不育系116A和Z11A为母本,改良后的新型抗病杂交种垦优杂741R和华油杂706R表现良好,具有较强的抗性和优良的农艺性状。这项工作推进了油菜杂种优势利用及根肿病抗性育种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e697/9504965/126d0fa24a00/plants-11-02413-g001.jpg

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