He Yan, Lu Lu, Peng Chao, Li Huilin, Zhang Jing, Li Run, Zhou Caiquan
Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong, 637002, China.
Institute of Ecology, China West Normal University, Nanchong, 637009, China.
AMB Express. 2020 May 6;10(1):86. doi: 10.1186/s13568-020-01018-2.
Pennisetum sinese Roxb (P. sinese) is an efficient and economic energy crop for its high productivity, and has been well studied in its application in phytoremediation and fodder production. However, little is known about how P. sinese plantation and fermented manures of P. sinese-feed livestock affect the composition of soil bacterial and fungal communities. In this study, 16S rRNA/ITS1 gene-based Illumina Miseq sequencing was employed to compare the bacterial and fungal community structure among soils that had been subjected to uncultivated control (CK), 2-year P. sinese plantation (P), and P. sinese plantation combined with the use of organic manures (P-OM) in a "P. sinese-breeding industry" ecological agriculture farm. The results found microbial communities were altered by P. sinese plantation and fertilization. The P. sinese plantation resulted in increased Actinobacteria and Planctomycetes abundance. Comparatively, significant increased abundance of Chloroflexi, Firmicutes, Nitrospirae, and Euryarchaeota, and genes related with nitrogen and carbon metabolic pathways based on PICRUSt prediction was observed in P-OM soil. Fungal compositions suggested a markedly increased abundance of Ascomycota in P soil. Potential organic matter decomposers Candida, Thermoascus, and Aspergillus were enriched in P soil, indicating the enhanced role of fungi in litter decomposition. Redundancy analysis suggested that soil properties (NH-N, total nitrogen, organic matter content, and soil water content) significantly correlated with the changes of microbial compositions (P < 0.05). These results highlight the divergence of microbial communities occurs during P. sinese-based plantation, implying functional diversification of soil ecosystem in P. sinese fields.
狼尾草是一种高产且经济高效的能源作物，在植物修复和饲料生产中的应用已得到充分研究。然而，关于狼尾草种植及其饲料家畜的发酵粪便如何影响土壤细菌和真菌群落的组成，人们知之甚少。在本研究中，我们采用基于16S rRNA/ITS1基因的Illumina Miseq测序技术，比较了在一个“狼尾草养殖产业”生态农业农场中，未耕种对照（CK）、2年狼尾草种植（P）以及狼尾草种植结合有机肥使用（P-OM）的土壤中细菌和真菌群落结构。结果发现，狼尾草种植和施肥改变了微生物群落。狼尾草种植导致放线菌和浮霉菌丰度增加。相比之下，在P-OM土壤中，绿弯菌门、厚壁菌门、硝化螺旋菌门和广古菌门的丰度显著增加，并且基于PICRUSt预测，与氮和碳代谢途径相关的基因也有所增加。真菌组成表明，P土壤中子囊菌门的丰度显著增加。潜在的有机物分解者念珠菌属、嗜热ascus属和曲霉属在P土壤中富集，表明真菌在凋落物分解中的作用增强。冗余分析表明，土壤性质（铵态氮、总氮、有机质含量和土壤含水量）与微生物组成的变化显著相关（P < 0.05）。这些结果突出了基于狼尾草种植过程中微生物群落的差异，这意味着狼尾草田地土壤生态系统的功能多样化。
