State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, West 130 Changjiang Road, Hefei, 230036 Anhui, People's Republic of China.
J Agric Food Chem. 2020 Jun 3;68(22):6221-6236. doi: 10.1021/acs.jafc.0c01833. Epub 2020 May 21.
Based on the abundance of taste compounds in leaves at different leaf positions on the same shoot, green tea made from one bud and one leaf, or even just one bud, has the best quality. To elucidate the mechanism underlying the regulation of the biosynthesis of these compounds, we profiled the metabolome, transcriptome, sRNA, degradome, and WGCNA using leaves from five leaf positions of shoots. Through this analysis, we found 139 miRNA-target pairs related to taste compound biosynthesis and 96 miRNA-target pairs involved in phytohormone synthesis or signal transduction. Moreover, miR166-, miR169-, IAA, ZA, ABA, and JA were positively related to the accumulation of gallated catechin, caffeine, and theanine. However, miR396-, miR393-, miR156-, and SA were negatively correlated with these compounds. Among these important pairs, the miR396- and miR156- pairs were further validated by using qRT-PCR, Northern blots, and cotransformation. This is the first report describing that miRNA-TF pairs and phytohormones might synergistically regulate the biosynthesis of taste compounds in the leaves of tea plants.
基于同一枝条上不同叶位叶片中味觉化合物的丰富度，用一芽一叶甚至仅仅一芽制成的绿茶品质最佳。为了阐明这些化合物生物合成调控的机制，我们利用枝条上五个叶位的叶片对代谢组、转录组、sRNA、降解组和 WGCNA 进行了分析。通过这项分析，我们发现了 139 对与味觉化合物生物合成相关的 miRNA-靶对和 96 对参与植物激素合成或信号转导的 miRNA-靶对。此外，miR166、miR169、IAA、ZA、ABA 和 JA 与没食子儿茶素、咖啡因和茶氨酸的积累呈正相关。然而，miR396、miR393、miR156 和 SA 与这些化合物呈负相关。在这些重要的对中，miR396 和 miR156 对通过 qRT-PCR、Northern blot 和共转化进一步得到验证。这是首次报道 miRNA-TF 对和植物激素可能协同调节茶树叶片中味觉化合物的生物合成。
