Key Laboratory of Horticulture Plant Biology, Ministry of Education, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
J Agric Food Chem. 2021 Feb 17;69(6):2001-2012. doi: 10.1021/acs.jafc.0c07440. Epub 2021 Feb 4.
This study investigated the regulatory relationship between important flavor compounds and microRNA (miRNA) in nine different tissues of tea plant by analyzing the related metabolites, small RNAs (sRNAs), degradome, and coexpression network. A total of 272 differential expressed miRNAs (DEmiRNAs) were obtained, including 198 conserved miRNAs and 74 novel miRNAs. Meanwhile, the expression patterns of miR159-, miR167-, and miR396- pairs were investigated by quantitative real-time polymerase chain reaction (qRT-PCR) and the target sites were verified by 5'RNA ligase-mediated RACE (5' RLM-RACE). Further coexpression analysis showed that the content of gallated catechins was significantly and negatively correlated with the expression of miR156, but positively correlated with the expression of miR166 and miR172. Additionally, the expression of miR169a, miR169l, and miR319h was shown to be positively correlated with the content of nongallated catechins and the experssion levels of , , and . Moreover, important volatile compounds, such as linalool, geraniol, and 2-phenylethanol, were found to be highly positively correlated with the expression of miR171o, miRN71a, miRN71b, miRN71c, and miRN71d. Our data indicate that these miRNAs may play important roles in regulating the biosynthesis of flavor compounds in different tissues of tea plant.
本研究通过分析相关代谢物、小 RNA（sRNA）、降解组和共表达网络，研究了 9 种茶树不同组织中重要风味化合物与 microRNA（miRNA）的调控关系。共获得 272 个差异表达 miRNA（DEmiRNA），包括 198 个保守 miRNA 和 74 个新 miRNA。同时，通过定量实时聚合酶链反应（qRT-PCR）对 miR159-、miR167-和 miR396- 对的表达模式进行了研究，并通过 5'RNA 连接酶介导的 RACE（5'RLM-RACE）验证了靶位点。进一步的共表达分析表明，没食子酰儿茶素的含量与 miR156 的表达呈显著负相关，但与 miR166 和 miR172 的表达呈正相关。此外，miR169a、miR169l 和 miR319h 的表达与非没食子酰儿茶素的含量呈正相关，与 、 和 的表达水平呈正相关。此外，一些重要的挥发性化合物，如芳樟醇、香叶醇和 2-苯乙醇，与 miR171o、miRN71a、miRN71b、miRN71c 和 miRN71d 的表达呈高度正相关。我们的数据表明，这些 miRNA 可能在调控茶树不同组织中风味化合物的生物合成中发挥重要作用。
