Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Fish Physiol Biochem. 2020 Jun;46(3):777-786. doi: 10.1007/s10695-019-00716-4. Epub 2020 May 7.
Grape seed, as a main source of polyphenols, has many nutritional and medicinal properties in humans. In the current study, the effects of dietary ethanolic grape seed extract (GSE) on the growth performance, antioxidant activity, and some biochemical parameters in rainbow trout were investigated. Ninety fish (initial weight 78.47 g) were randomly distributed among nine cement tanks (1.8 m × 0.22 m × 0.35 m) with 10 fish per tank. Three experimental diets containing either 0, 10, or 50 g kg GSE were prepared and each diet was randomly assigned to three tanks of fish for 60 days. Results showed that feeding GSE enhanced some growth parameters including the specific growth rate and condition factor in comparison with the control group. Among different serum metabolites, the glucose levels in treatment groups significantly decreased compared to the control group. The total product of lipid peroxidation indicated as malondialdehyde significantly decreased in both the GSE-added treatment groups. The gene expression related to the antioxidant enzymes, catalase, glutathione peroxidase 1, and glutathione S-transferase A, were upregulated in the intestine of fish that received a low dose of GSE. The results of the current study suggest that GSE, especially at 10 g kg, diet had the potential to improve (1) specific growth rate and condition factor, (2) biochemical parameters including glucose and lipid peroxidation product, and (3) upregulated the expression of antioxidant genes including catalase, glutathione peroxidase 1, and glutathione S-transferase A in rainbow trout.
葡萄籽作为多酚的主要来源,对人体具有许多营养和药用特性。在本研究中,研究了饮食中含乙醇的葡萄籽提取物(GSE)对虹鳟生长性能、抗氧化活性和一些生化参数的影响。将 90 条鱼(初始体重 78.47g)随机分配到 9 个水泥池中(1.8m×0.22m×0.35m),每个池中有 10 条鱼。制备了三种含有 0、10 或 50g kg GSE 的实验饲料,每种饲料随机分配到三个鱼池中的 30 条鱼,为期 60 天。结果表明,与对照组相比,GSE 喂养可提高一些生长参数,包括特定生长率和体况系数。在不同的血清代谢物中,与对照组相比,GSE 处理组的葡萄糖水平显著降低。丙二醛作为总脂质过氧化产物在添加 GSE 的处理组中均显著降低。在接受低剂量 GSE 的鱼的肠道中,与抗氧化酶相关的基因表达,如过氧化氢酶、谷胱甘肽过氧化物酶 1 和谷胱甘肽 S-转移酶 A,均上调。本研究结果表明,GSE,特别是在 10g kg 的饮食中,有可能改善(1)特定生长率和体况系数,(2)包括葡萄糖和脂质过氧化产物在内的生化参数,以及(3)上调虹鳟鱼的抗氧化基因表达,包括过氧化氢酶、谷胱甘肽过氧化物酶 1 和谷胱甘肽 S-转移酶 A。