College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Henan Agricultural University, Zhengzhou 450002, China.
Mol Immunol. 2020 Aug;124:180-189. doi: 10.1016/j.molimm.2020.06.004. Epub 2020 Jun 24.
Stress is a physiological manifestation of the body's defense against adverse effects of external environment, but the molecular regulatory mechanism of stress effects on immune function of poultry has not been fully clarified. In this study, 7-day-old Chinese local breed Gushi cocks were used as model animal, and the stress model was successfully constructed by adding corticosterone (CORT) 30 mg/kg basic diet for 7 days. The spleen transcriptomes of the control group (B_S group) and the stress model group (C_S group) was determined by high-throughput mRNA sequencing (RNA-Seq) technology, and a total of 269 significantly differentially expressed genes (SDEGs) were obtained (Padj < 0.05, |FC| ≥ 2 and FPKM > 1). Compared with B_S group, there were 140 significantly up-regulated genes and 129 significantly down-regulated genes in C_S group. The immune/stress-related Gene Ontology (GO) terms included positive regulation of T cell mediated immunity, chemokine-mediated signaling pathway, T cell mediated immunity and so on. The SDEGs such as IL8L1, HSPA8, HSPA2, RSAD2, CCR8L and DMB1 were involved in these GO terms. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis showed that the SDEGs participated in many immune-related signaling pathways. The immune-related genes HSPA2, HSPA8, HSP90AA1, HSPH1 and HERPUD1 were enriched in Protein processing in endoplasmic reticulum pathway, IL8L1, CXCL13L2, CCR6, LEPR, CCR9 and CCR8L were enriched in Cytokine-cytokine receptor interaction pathway. The protein-protein interactions (PPI) analysis showed HSPA8, HSPA2 and IL8L1 as key core nodes had 7 interactions and may play important roles in the regulation of CORT-induced stress effects on immune function. The data onto this study enriched the genomic study of stress effects on immune function, and provided unique insights into the molecular mechanism of stress effects on immune function, and the genes identified in this study can be candidates for future research on stress response.
应激是机体对外界环境不良影响产生防御的一种生理表现，但应激对禽类免疫功能影响的分子调控机制尚未完全阐明。本研究以 7 日龄中国地方品种固始鸡为模型动物，通过在基础饲粮中添加 30mg/kg 皮质酮（CORT）构建应激模型。采用高通量 mRNA 测序（RNA-Seq）技术测定对照组（B_S 组）和应激模型组（C_S 组）的脾脏转录组，共获得 269 个显著差异表达基因（SDEGs）（Padj<0.05，|FC|≥2 且 FPKM>1）。与 B_S 组相比，C_S 组中有 140 个基因显著上调，129 个基因显著下调。与免疫/应激相关的基因本体论（GO）术语包括 T 细胞介导的免疫的正调控、趋化因子介导的信号通路、T 细胞介导的免疫等。IL8L1、HSPA8、HSPA2、RSAD2、CCR8L 和 DMB1 等 SDEGs 涉及这些 GO 术语。京都基因与基因组百科全书（KEGG）富集分析显示，SDEGs 参与了许多免疫相关信号通路。免疫相关基因 HSPA2、HSPA8、HSP90AA1、HSPH1 和 HERPUD1 富集于内质网蛋白加工途径，IL8L1、CXCL13L2、CCR6、LEPR、CCR9 和 CCR8L 富集于细胞因子-细胞因子受体相互作用途径。蛋白质-蛋白质相互作用（PPI）分析显示，HSPA8、HSPA2 和 IL8L1 作为关键核心节点有 7 个相互作用，可能在 CORT 诱导的应激对免疫功能的影响调节中发挥重要作用。本研究的数据丰富了应激对免疫功能影响的基因组研究，为应激对免疫功能影响的分子机制提供了独特的见解，本研究中鉴定的基因可以作为应激反应相关研究的候选基因。
