1College of Animal Science and Technology,Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee,Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,No. 1 Weigang Road,Nanjing 210095,P.R. China.
2Ginling College,Nanjing Normal University,Nanjing 210097,P.R. China.
Animal. 2018 Dec;12(12):2561-2570. doi: 10.1017/S1751731118000903. Epub 2018 May 3.
The oxidative study has always been particularly topical in poultry science. However, little information about the occurrence of cellular apoptosis and autophagy through the reactive oxygen species (ROS) generation in nuclear factor-κB (NF-κB) signal pathway was reported in the liver of broilers exposed to hydrogen peroxide (H2O2). So we investigated the change of growth performance of broilers exposed to H2O2 and further explored the occurrence of apoptosis and autophagy, as well as the expression of NF-κB in these signaling pathways in the liver. A total of 320 1-day-old Arbor Acres male broiler chickens were raised on a basal diet and randomly divided into five treatments which were arranged as non-injected treatment (Control), physiological saline (0.75%) injected treatment (Saline) and H2O2 treatments (H2O2(0.74), H2O2(1.48) and H2O2(2.96)) received an intraperitoneal injection of H2O2 with 0.74, 1.48 and 2.96 mM/kg BW. The results showed that compared to those in the control and saline treatments, 2.96 mM/kg BW H2O2-treated broilers exhibited significantly higher feed/gain ratio at 22 to 42 days and 1 to 42 days, ROS formation, the contents of oxidation products, the mRNA expressions of caspases (3, 6, 8), microtubule-associated protein 1 light chain 3 (LC3)-II/LC3-I, autophagy-related gene 6, Bcl-2 associated X and protein expressions of total caspase-3 and total LC3-II, and significantly lower BW gain at 22 to 42 days and 1 to 42 days, the activities of total superoxide dismutase and glutathione peroxidase, the expression of NF-κB in the liver. Meanwhile, significantly higher feed/gain ratio at 1 to 42 days, ROS formation, the contents of protein carbonyl and malondialdehyde, the mRNA expression of caspase-3 and the protein expressions of total caspase-3 and total LC3-II, as well as significantly lower BW gain at 22 to 42 days and 1 to 42 days were observed in broilers received 1.48 mM/kg BW H2O2 treatment than those in control and saline treatments. These results indicated that oxidative stress induced by H2O2 had a negative effect on histomorphology and redox status in the liver of broilers, which was associated with a decline in growth performance of broilers. This may attribute to apoptosis and autophagy processes triggered by excessive ROS that suppress the NF-κB signaling pathway.
过氧化氢诱导肉鸡肝脏氧化应激及其对核因子-κB 信号通路相关凋亡和自噬的影响
本研究旨在探讨过氧化氢（H2O2）诱导肉鸡肝脏氧化应激及其对核因子-κB（NF-κB）信号通路相关凋亡和自噬的影响。将 320 只 1 日龄雄性爱拔益加（Arbor Acres）肉鸡随机分为 5 个处理组，分别为非注射对照组（Control）、生理盐水（0.75%）对照组（Saline）和 H2O2 处理组（H2O2（0.74）、H2O2（1.48）和 H2O2（2.96）），每组 64 个重复，每个重复 1 只鸡。H2O2 处理组鸡分别接受 0.74、1.48 和 2.96 mM/kg BW 的 H2O2 腹腔注射。结果显示，与对照组和生理盐水对照组相比，2.96 mM/kg BW H2O2 处理组肉鸡在 22 至 42 日龄和 1 至 42 日龄时的饲料/增重比显著升高，ROS 生成、氧化产物含量、半胱天冬氨酸蛋白酶（caspase）3、6、8（caspase-3、6、8）、微管相关蛋白 1 轻链 3（microtubule-associated protein 1 light chain 3，LC3）-II/LC3-I、自噬相关基因 6（autophagy-related gene 6，Atg6）、Bcl-2 相关 X（Bcl-2 associated X，Bax）和总半胱天冬氨酸蛋白酶-3（total caspase-3）和总 LC3-II 蛋白表达水平显著升高，22 至 42 日龄和 1 至 42 日龄时的体重增重显著降低，总超氧化物歧化酶和谷胱甘肽过氧化物酶活性以及 NF-κB 的表达水平显著降低。同时，1.48 mM/kg BW H2O2 处理组肉鸡在 1 至 42 日龄时的饲料/增重比、ROS 生成、蛋白质羰基和丙二醛含量、caspase-3 mRNA 表达以及总 caspase-3 和总 LC3-II 蛋白表达水平均显著高于对照组和生理盐水对照组，22 至 42 日龄和 1 至 42 日龄时的体重增重均显著低于对照组和生理盐水对照组。这些结果表明，H2O2 诱导的氧化应激对肉鸡肝脏的组织形态和氧化还原状态产生了负面影响，导致肉鸡生长性能下降。这可能归因于过量 ROS 触发的凋亡和自噬过程抑制了 NF-κB 信号通路。
