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WFhb1-1 在小麦抗赤霉病中发挥重要作用。

WFhb1-1 plays an important role in resistance against Fusarium head blight in wheat.

机构信息

Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA.

College of Agronomy, Shandong Agricultural University, Taian, Shandong, 271018, China.

出版信息

Sci Rep. 2020 May 8;10(1):7794. doi: 10.1038/s41598-020-64777-9.

DOI:10.1038/s41598-020-64777-9
PMID:32385328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7210279/
Abstract

Fusarium head blight (FHB) is a severe disease of wheat (Triticum aestivum L.). Qfhb1 is the most important quantitative trait locus (QTL) for FHB resistance. We previously identified wheat gene WFhb1-1 (aka WFhb1-c1) as a candidate for FHB resistance gene. Here we report that WFhb1-1 has been cloned. The gene (GenBank # KU304333.1) consists of a single exon, encoding a putative membrane protein of 127 amino acids. WFhb1-1 protein produced in Pichia pastoris inhibits growth of both F. graminearum and P. pastoris in culture. Western Blotting with anti- WFhb1-1 antibody revealed a significant decrease (p < 0.01) in WFhb1-1 accumulation, 12 hours post Fusarium inoculation in non-Qfhb1-carrier wheat but not in Qfhb1-carrier wheat. Overexpressing WFhb1-1 in non-Qfhb1-carrier wheat led to a significant decrease (p < 0.01) in Fusarium-damaged rachis rate, Fusarium-diseased kernel rate and DON content in harvested kernels, while silencing WFhb1-1 in Qfhb1-carrier wheat resulted in a significant increase (p < 0.01) in FHB severity. Therefore, WFhb1-1 is an important FHB resistance gene with a potential antifungal function and probably a key functional component of Qfhb1 in wheat. A model regarding how WFhb1-1 functions in FHB resistance/susceptibility is hypothesized and discussed.

摘要

镰刀菌穗腐病（FHB）是小麦（Triticum aestivum L.）的一种严重病害。Qfhb1 是 FHB 抗性的最重要的数量性状位点（QTL）。我们之前已经鉴定了小麦基因 WFhb1-1（又名 WFhb1-c1）作为 FHB 抗性基因的候选基因。在这里，我们报告了 WFhb1-1 已经被克隆。该基因（GenBank # KU304333.1）由一个单一的外显子组成，编码一个 127 个氨基酸的假定膜蛋白。在毕赤酵母中表达的 WFhb1-1 蛋白在培养物中抑制禾谷镰刀菌和巴斯德毕赤酵母的生长。用抗 WFhb1-1 抗体进行的 Western Blotting 显示，在非 Qfhb1 载体小麦中，接种镰刀菌 12 小时后 WFhb1-1 的积累显著减少（p<0.01），但在 Qfhb1 载体小麦中则没有。在非 Qfhb1 载体小麦中过表达 WFhb1-1 导致镰刀菌损伤穗率、镰刀菌病籽粒率和收获籽粒中 DON 含量显著降低（p<0.01），而在 Qfhb1 载体小麦中沉默 WFhb1-1 则导致 FHB 严重度显著增加（p<0.01）。因此，WFhb1-1 是一个重要的 FHB 抗性基因，具有潜在的抗真菌功能，可能是小麦 Qfhb1 的关键功能组成部分。提出并讨论了关于 WFhb1-1 在 FHB 抗性/易感性中作用的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/8881148a360f/41598_2020_64777_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/3f67a3d16c58/41598_2020_64777_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/ce47068758b8/41598_2020_64777_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/3ea3f1bf07c4/41598_2020_64777_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/0d697176f1dc/41598_2020_64777_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/4a16bd12c1f0/41598_2020_64777_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/946275695316/41598_2020_64777_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/467035422e63/41598_2020_64777_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/8881148a360f/41598_2020_64777_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/3f67a3d16c58/41598_2020_64777_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/ce47068758b8/41598_2020_64777_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/3ea3f1bf07c4/41598_2020_64777_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/0d697176f1dc/41598_2020_64777_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/4a16bd12c1f0/41598_2020_64777_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/946275695316/41598_2020_64777_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/467035422e63/41598_2020_64777_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181b/7210279/8881148a360f/41598_2020_64777_Fig8_HTML.jpg

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