Department of Pathology, Immunology and Laboratory Medicine, Center for Immunity and Inflammation, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
J Infect Dis. 2020 Sep 14;222(8):1400-1404. doi: 10.1093/infdis/jiaa251.
Staphylococcus aureus is a leading cause of pneumonia. We show here that the ClpXP protease involved in protein turnover is important for pathogenesis in a murine model of acute pneumonia. Staphylococcus aureus lacking this protease is attenuated in vivo, being rapidly cleared from the airway and leading to decreased immune cell influx and inflammation. Characterization of defined mutations in vitro identified defects in intracellular survival and protection against neutrophil killing. Our results further expand on what is known about ClpXP in the pathogenesis of S. aureus to include the respiratory tract.
金黄色葡萄球菌是肺炎的主要病因。我们在此表明，参与蛋白质周转的 ClpXP 蛋白酶对于急性肺炎的小鼠模型中的发病机制很重要。缺乏这种蛋白酶的金黄色葡萄球菌在体内减毒，迅速从气道中清除，导致免疫细胞浸润和炎症减少。体外鉴定的明确突变的特征是细胞内存活缺陷和对中性粒细胞杀伤的保护缺陷。我们的研究结果进一步扩展了 ClpXP 在金黄色葡萄球菌发病机制中的已知功能，包括呼吸道。
