College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
College of Life Sciences, Chongqing Normal University, Chongqing 401331, China.
Int J Biol Macromol. 2020 Sep 15;159:231-235. doi: 10.1016/j.ijbiomac.2020.04.269. Epub 2020 May 5.
GCP, a polysaccharide produced by endophytic fungus Chaetomium globosum CGMCC 6882, was found to be antibacterial against Staphylococcus aureus via disrupting cell permeability. However, the antibacterial mechanism of GCP has not been studied before. In present work, results showed that GCP could retard the growth of S. aureus by inducing the depolarization of cell membrane, decreasing the activity of Ca-Mg-ATPase on cell membrane, and increasing the accumulation of calcium ions in cytoplasm. Moreover, we found that GCP could also inhibit the synthesis of whole cell proteins of S. aureus. Overall, this study proved that the antibacterial mechanism of GCP could be diversified and more studies are needed in the investigation of the antibacterial mechanisms of various polysaccharides.
GCP 是一种由内生真菌 Chaetomium globosum CGMCC 6882 产生的多糖，通过破坏细胞通透性被发现对金黄色葡萄球菌具有抗菌作用。然而，GCP 的抗菌机制以前尚未研究过。在本工作中，结果表明 GCP 通过诱导细胞膜去极化、降低细胞膜上 Ca-Mg-ATP 酶的活性以及增加细胞质中钙离子的积累来抑制金黄色葡萄球菌的生长。此外，我们发现 GCP 还可以抑制金黄色葡萄球菌的整个细胞蛋白质的合成。总的来说，这项研究证明了 GCP 的抗菌机制可能是多样化的，需要对各种多糖的抗菌机制进行更多的研究。
