一种从内生真菌Chaetomium sp. M336中分离得到的具有抗菌和细胞毒性活性的新型表硫代二氧哌嗪。

A new epipolythiodioxopiperazine with antibacterial and cytotoxic activities from the endophytic fungus Chaetomium sp. M336.

作者信息

Yu Fei-Xue, Chen Yao, Yang Yin-He, Li Guo-Hong, Zhao Pei-Ji

机构信息

a State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, and Key Laboratory for Microbial Resources of the Ministry of Education , Yunnan University , Kunming , China.

b Faculty of medicine , Kunming University of Science and Technology , Kunming , China.

出版信息

Nat Prod Res. 2018 Mar;32(6):689-694. doi: 10.1080/14786419.2017.1338285. Epub 2017 Jun 12.

DOI:10.1080/14786419.2017.1338285

PMID:28602097

Abstract

A new compound 6-formamide-chetomin (1) together with chetomin (2) was isolated from solid fermentation products of the endophytic fungus Chaetomium sp. M336, which were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HR-ESI-MS experiments. The bioassay result showed that compound 1 had strong antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium ATCC 6539 and Enterococcus faecalis with minimum inhibitory concentration (MIC) value of 0.78 μg/mL; meanwhile it exhibited strong cytotoxicity with IC values of 21.6-27.1 nM against cell lines HeLa, SGC-7901 and A549.

摘要

从内生真菌Chaetomium sp. M336的固体发酵产物中分离出一种新化合物6-甲酰胺基-赭曲霉素（1）和赭曲霉素（2），通过广泛的光谱分析对其进行了鉴定，包括一维和二维核磁共振以及高分辨电喷雾电离质谱实验。生物活性测试结果表明，化合物1对大肠杆菌、金黄色葡萄球菌、鼠伤寒沙门氏菌ATCC 6539和粪肠球菌具有较强的抗菌活性，最低抑菌浓度（MIC）值为0.78μg/mL；同时，它对HeLa、SGC-7901和A549细胞系表现出较强的细胞毒性，IC值为21.6 - 27.1 nM。