Lee Sung Won, Park Hyun Jung, Pei Yihua, Ye Yoon, Hong Seokmann
Department of Integrative Bioscience and Biotechnology, Institute of Anticancer Medicine Development, Sejong University, Seoul 05006, Republic of Korea.
Department of Industrial and Physical Pharmacy, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA.
Int J Biol Macromol. 2020 May 6. doi: 10.1016/j.ijbiomac.2020.05.017.
Zwitterionic chitosan (ZWC), a water-soluble succinylated chitosan derivative, has anti-inflammatory activities with therapeutic effects on sepsis and colitis. However, it remains unknown whether ZWC has any influence on skin inflammation. Here, we investigated the role of ZWC in the tape-stripping-induced acute skin inflammation model. Topical application of ZWC to the wounded area significantly reduced skin lesion compared with PBS controls. Since tape-stripping-induced skin inflammation is mediated by neutrophils, we examined if ZWC has any suppressive effects on neutrophil's function. ZWC treatment downregulated the skin recruitment of neutrophils, subsequently reducing inflammatory responses by keratinocytes. ZWC also suppressed LPS-induced inflammatory responses of neutrophils in vitro, indicating the direct effect of ZWC on neutrophils. Moreover, such anti-inflammatory effects of ZWC extended to other immune cells such as basophils in the spleen. Overall, our results support that ZWC may be used as a therapeutic material to control acute skin inflammation.
两性离子壳聚糖（ZWC）是一种水溶性琥珀酰化壳聚糖衍生物，具有抗炎活性，对败血症和结肠炎有治疗作用。然而，ZWC对皮肤炎症是否有任何影响尚不清楚。在此，我们研究了ZWC在胶带剥离诱导的急性皮肤炎症模型中的作用。与PBS对照组相比，在伤口部位局部应用ZWC可显著减少皮肤损伤。由于胶带剥离诱导的皮肤炎症是由中性粒细胞介导的，我们研究了ZWC是否对中性粒细胞的功能有任何抑制作用。ZWC处理下调了中性粒细胞在皮肤中的募集，随后减少了角质形成细胞的炎症反应。ZWC还在体外抑制了LPS诱导的中性粒细胞炎症反应，表明ZWC对中性粒细胞有直接作用。此外，ZWC的这种抗炎作用还扩展到了其他免疫细胞，如脾脏中的嗜碱性粒细胞。总体而言，我们的结果支持ZWC可作为一种治疗材料来控制急性皮肤炎症。
