海藻对寨卡病毒的抗病毒活性。

Antiviral Activity of Seaweed against ZIKV.

机构信息

Laboratório de Imunovirologia, Programa de Pós-graduação em Ciências e Biotecnologia, Programa de Pós-Graduação em Biotecnologia Marinha, Departamento de Imunobiologia, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil.

Laboratório de Virologia Molecular e Biotecnologia Marinha, Programa de Pós-graduação em Ciências e Biotecnologia, Programa de Pós-Graduação em Biotecnologia Marinha, Programa de Pós-graduação em Neurologia/Neurociência, Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil.

出版信息

Curr Top Med Chem. 2024;24(18):1589-1598. doi: 10.2174/0115680266294503240513044930.

DOI:10.2174/0115680266294503240513044930

PMID:38797894

Abstract

INTRODUCTION

Zika virus (ZIKV) is a flavivirus transmitted through the bites of infected Aedes mosquitoes. These viruses can also be transmitted through sexual contact, vertical transmission, and possibly transfusion. Most cases are asymptomatic, but symptoms can include rash, conjunctivitis, fever, and arthralgia, which are characteristic of other arboviruses. Zika infection can lead to complications such as microcephaly, miscarriage, brain abnormalities, and Guillain-Barré syndrome (GBS).

OBJECTIVE

The aim is to determine the inhibitory potential of the algae on ZIKV replication.

METHODOLOGY

Cytotoxicity experiments were performed using Vero cells to determine the CC, and ZIKV replication inhibition assays (ATCC VR-1839™) were conducted to determine the EC. The mechanism of action was also studied to assess any synergistic effect with Ribavirin.

RESULTS

demonstrated low toxicity with a CC of 423 μg/mL and a potent effect on ZIKV replication with an EC of 0.65 μg/mL and a Selectivity Index (SI) of 651, indicating the extract's safety. Virucidal effect assays were carried out to evaluate the possible mechanism of action, and the compound addition time was studied, showing the potential to delay the treatment of infected cells by up to 6 hours. A potential synergistic effect was observed when extract was combined with suboptimal concentrations of Ribavirin, resulting in 99% inhibition of viral replication.

CONCLUSION

Our data demonstrate the significant potential of extract and highlight the need for further studies to investigate its mechanism of action. We propose this extract as a potential anti-Zika compound.

摘要

简介

Zika 病毒（ZIKV）是一种黄病毒，通过受感染的埃及伊蚊叮咬传播。这些病毒也可以通过性接触、垂直传播和可能的输血传播。大多数病例无症状，但症状可能包括皮疹、结膜炎、发热和关节痛，这些都是其他虫媒病毒的特征。Zika 感染可导致小头畸形、流产、脑异常和格林-巴利综合征（GBS）等并发症。

目的

目的是确定藻类对 ZIKV 复制的抑制潜力。

方法

使用 Vero 细胞进行细胞毒性实验以确定 CC，并用 ZIKV 复制抑制测定法（ATCC VR-1839™）进行实验以确定 EC。还研究了作用机制，以评估与利巴韦林的协同作用。

结果

显示出低毒性，CC 为 423μg/ml，对 ZIKV 复制具有强大的作用，EC 为 0.65μg/ml，选择性指数（SI）为 651，表明提取物的安全性。进行了病毒杀伤作用实验以评估可能的作用机制，并研究了化合物添加时间，表明有可能将感染细胞的治疗时间延迟长达 6 小时。当与利巴韦林的亚最佳浓度联合使用时，观察到潜在的协同作用，导致病毒复制抑制率达到 99%。