Kaplan Ben, Merdler Uri, Szklanny Ariel A, Redenski Idan, Guo Shaowei, Bar-Mucha Zemach, Michael Noah, Levenberg Shulamit
Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel; Bruce Rapaport Faculty of Medicine, Technion- Israel Institute of Technology, Haifa, Israel.
Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel.
Biomaterials. 2020 Aug;251:120062. doi: 10.1016/j.biomaterials.2020.120062. Epub 2020 Apr 22.
Biodegradable polyesters have been extensively used for preparation of nerve guidance scaffolds, due to their high biocompatibility and defined degradation periods. However, conventional methods for fabrication of porous polyester scaffolds provide limited control over shape and micro-architecture. Here, a fabrication procedure based on 3D printing was developed to generate highly ordered and anatomically personalized, polyester scaffolds for soft tissue regeneration. Scaffolds composed of Poly-lactic-glycolic acid (PLGA) and poly-L-lactic acid (PLLA) were specifically customized for nerve injuries. This was obtained by using an oriented multi-layer printing pattern which established a linear structure in the fabricated scaffolds to match the aligned topography of nerve tissues. The oriented scaffold was shown to guide regenerating axons to linear conformations and support growth of induced pluripotent stem cell-derived neurons in vitro and in vivo in a model of spinal cord injury. The described scaffolds may advance the field of nerve regeneration. Furthermore, modifications could be integrated to generate soft implants for various types of tissues.
由于具有高生物相容性和明确的降解周期，可生物降解聚酯已被广泛用于制备神经导向支架。然而，传统的多孔聚酯支架制造方法对形状和微观结构的控制有限。在此，开发了一种基于3D打印的制造程序，以生成用于软组织再生的高度有序且符合解剖学个性化的聚酯支架。由聚乳酸-乙醇酸共聚物（PLGA）和聚-L-乳酸（PLLA）组成的支架是专门为神经损伤定制的。这是通过使用定向多层打印图案实现的，该图案在制造的支架中建立了线性结构，以匹配神经组织的排列地形。在脊髓损伤模型中，定向支架在体外和体内均显示出可引导再生轴突形成线性构象，并支持诱导多能干细胞衍生神经元的生长。所述支架可能推动神经再生领域的发展。此外，可以进行改进以生成用于各种组织类型的软性植入物。
