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转录组分析揭示了添加L-丙氨酸激活后虫草素网络的灵活性。

Transcriptome Analysis Reveals the Flexibility of Cordycepin Network in Activated by L-Alanine Addition.

作者信息

Chen Bai-Xiong, Wei Tao, Xue Ling-Na, Zheng Qian-Wang, Ye Zhi-Wei, Zou Yuan, Yang Yi, Yun Fan, Guo Li-Qiong, Lin Jun-Fang

机构信息

Institute of Food Biotechnology & College of Food Science, South China Agricultural University, Guangzhou, China.

Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, China.

出版信息

Front Microbiol. 2020 Apr 24;11:577. doi: 10.3389/fmicb.2020.00577. eCollection 2020.

DOI:10.3389/fmicb.2020.00577
PMID:32390960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7193312/
Abstract

Cordycepin, isolated from the traditional medicinal fungus , has gained much attention due to its various clinical functions. Previous reports of L-alanine addition could significantly improve cordycepin production, but the molecular mechanism remains unknown. In this study, transcriptome analysis of with doubled cordycepin production induced by L-alanine addition provides an insight into the flexibility of the cordycepin network. The biopathways of energy generation and amino acid conversion were activated so that cordycepin substrate generation was consequently improved. Specific genes of rate-limiting enzymes in these pathways, as well as related transcription factors, were figured out. Two key Zn2Cys6-type transcription factors CmTf1 and CmTf2 were verified to play the roles of doubling the cordycepin production by overexpression of their coding genes in wild type. These results provide a complete map of the cordycepin network in with a distinct understanding of the flexibility of joints, giving a better foundation for increasing cordycepin yield and strain breeding in the future.

摘要

从传统药用真菌中分离出的虫草素，因其多种临床功能而备受关注。先前有报道称添加L-丙氨酸可显著提高虫草素产量，但其分子机制尚不清楚。在本研究中，对添加L-丙氨酸诱导虫草素产量翻倍的进行转录组分析，有助于深入了解虫草素网络的灵活性。能量生成和氨基酸转化的生物途径被激活，从而提高了虫草素底物的生成。确定了这些途径中限速酶的特定基因以及相关转录因子。通过在野生型中过表达其编码基因，验证了两个关键的Zn2Cys6型转录因子CmTf1和CmTf2在使虫草素产量翻倍中所起的作用。这些结果提供了虫草素网络的完整图谱，对其连接灵活性有了清晰的认识，为未来提高虫草素产量和菌株育种奠定了更好的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/853f85cda25e/fmicb-11-00577-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/7c0b14a833ab/fmicb-11-00577-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/c233b018e6ae/fmicb-11-00577-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/f00dce43a21e/fmicb-11-00577-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/caca4ab7c51d/fmicb-11-00577-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/4cfa2eda33e6/fmicb-11-00577-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/d5d08c983358/fmicb-11-00577-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/853f85cda25e/fmicb-11-00577-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/7c0b14a833ab/fmicb-11-00577-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/c233b018e6ae/fmicb-11-00577-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/f00dce43a21e/fmicb-11-00577-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/caca4ab7c51d/fmicb-11-00577-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/4cfa2eda33e6/fmicb-11-00577-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/d5d08c983358/fmicb-11-00577-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a9a/7193312/853f85cda25e/fmicb-11-00577-g007.jpg

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