Long Liangkun, Liu Zhen, Wang Yizhou, Lin Qunying, Ding Shaojun, Li Chuanhua, Deng Chunying
Jiangsu Co-Innovation Centre for Efficient Processing and Utilisation of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Jiangsu Key Lab for the Chemistry & Utilisation of Agricultural and Forest Biomass, Nanjing 210037, China.
Jiangsu Co-Innovation Centre for Efficient Processing and Utilisation of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2023 Nov;388:129742. doi: 10.1016/j.biortech.2023.129742. Epub 2023 Sep 19.
Cordycepin is an important active metabolite of Cordyceps militaris. Xylose, an attractive feedstock for producing chemicals through microbial fermentation, cannot be effectively utilised by many reported C. militaris strains. Herein, a xylose-utilising C. militaris strain 147 produced the highest level of cordycepin (3.03 g/L) in xylose culture. Xylose, alanine, and ammonium citrate were determined as the main affecting factors on the cordycepin production using a Plackett-Burman design. The combination of these factors was optimised using response surface methodology, and the maximal 6.54 g/L of cordycepin was produced by the fungus in the optimal medium. Transcriptome analysis revealed that xylose utilisation upregulated the transcriptional levels of genes participating in purine and energy metabolisms in the fungus, which may facilitate the formation of precursors for cordycepin biosynthesis. This investigation provides new insights into the efficient production of cordycepin and is conducive to the valorisation of biomass rich in xylose.
虫草素是蛹虫草的一种重要活性代谢产物。木糖是一种通过微生物发酵生产化学品的有吸引力的原料，但许多已报道的蛹虫草菌株不能有效利用它。在此，一株利用木糖的蛹虫草菌株147在木糖培养物中产生了最高水平的虫草素（3.03克/升）。使用Plackett-Burman设计确定木糖、丙氨酸和柠檬酸铵是影响虫草素产量的主要因素。利用响应面法对这些因素的组合进行了优化，该真菌在最佳培养基中产生了最大6.54克/升的虫草素。转录组分析表明，木糖利用上调了真菌中参与嘌呤和能量代谢的基因的转录水平，这可能有助于虫草素生物合成前体的形成。本研究为虫草素的高效生产提供了新的见解，并有助于富含木糖的生物质的增值利用。
