Shimadzu Research Laboratory (Shanghai) Co. Ltd., Shanghai 201206, People's Republic of China.
Genomics Research Center, Academia Sinica, Taipei 115, Taiwan.
Anal Chem. 2020 Jun 16;92(12):8356-8363. doi: 10.1021/acs.analchem.0c00868. Epub 2020 May 22.
In recent years, there has been a rapid increase in the use of counter-flow-type ion mobility spectrometers (IMS) due to their improved resolution and functionality. In this study, we developed a new type of counter-flow ion mobility device named the U-shaped mobility analyzer (UMA) for coupling with a mass spectrometer, where the analyte ions could travel along a gas flow in the first channel of the UMA device and then against a gas flow in the second channel of the device. Hence, a mobility band-pass filter was formed by setting different electric fields in the two channels, which enables high-resolution mobility selection of analyte ions. A resolution of ca. 180 was achieved for singly charged small organic molecules, and a resolution of up to ca. 370 was achieved for multiply charged +15 myoglobin. It was thus demonstrated that this filtering function can greatly enhance the dynamic range of an IMS-MS instrument, particularly favoring targeted analysis in complex matrices. Alternatively, the analyte ions could be operated in a so-called trap-scan mode in which ions were trapped first in one of the channels and released sequentially for mobility analysis with an even higher resolution (ca. 210 for singly charged small organic molecules and ca. 590 for +15 myoglobin). Overall, this new UMA device would enable many new applications in omics studies with its high resolution and dynamic range, especially when using the filter-scan mode for scrutinizing analytes with very low concentrations under high chemical backgrounds.
近年来，由于其分辨率和功能的提高，逆流型离子迁移谱（IMS）的使用迅速增加。在这项研究中，我们开发了一种新型的逆流离子迁移装置，命名为 U 形迁移分析器（UMA），用于与质谱仪耦合，其中分析物离子可以沿着 UMA 装置的第一通道中的气流移动，然后与装置的第二通道中的气流逆行。因此，通过在两个通道中设置不同的电场，可以形成一个迁移带通滤波器，从而实现对分析物离子的高分辨率迁移选择。对于单电荷小分子，可实现约 180 的分辨率，对于+15 肌红蛋白，可实现高达约 370 的分辨率。因此，证明这种滤波功能可以大大增强 IMS-MS 仪器的动态范围，特别是有利于在复杂基质中进行靶向分析。或者，分析物离子可以在所谓的“阱扫描模式”下操作，其中离子首先被捕获在一个通道中，并依次释放以进行迁移分析，分辨率更高（对于单电荷小分子约为 210，对于+15 肌红蛋白约为 590）。总的来说，这种新的 UMA 装置将凭借其高分辨率和动态范围，在组学研究中实现许多新的应用，特别是在使用滤波器扫描模式时，可以对高化学背景下浓度非常低的分析物进行详细分析。
