Department of Radiology, Xiangya Hospital, Central South University, Changsha, China.
Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, China.
Cancer Biother Radiopharm. 2021 Mar;36(2):211-219. doi: 10.1089/cbr.2019.3271. Epub 2020 May 8.
Hepatocellular carcinoma (HCC) is associated with high morbidity and mortality and has become the most frequently diagnosed liver cancer globally. Long noncoding RNAs have been widely studied because they exert essential functions in human diseases. The aim of the study is to explore the role and molecular regulatory mechanism of in HCC. Real-time quantitative polymerase chain reaction examined , , and mitochondrial ribosomal protein S18a () expression in HCC cells. Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry, JC-1, transwell, and Western blot assays uncovered the function of in HCC. RNA immunoprecipitation (RIP), RNA pull down, and luciferase reporter assays validated the association among , , and . Nuclear-cytoplasmic fractionation assay revealed the subcellular location of in HCC cells. was revealed to be upregulated in HCC tissue samples according to GEPIA database. Consistent results were recognized in HCC cell lines. Subsequently, loss-of-function assays confirmed that ablation depressed cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition process and inhibited tumor growth . Furthermore, the authors validated bound with in HCC. inversely regulated expression. Afterward, was identified to be a downstream target of . Ultimately, rescue assays manifested that upregulation could neutralize the attenuated effects resulting from deficiency. All in all, sponged to facilitate HCC progression through increasing expression. The findings highlight as a novel therapeutic target for HCC.
肝细胞癌（HCC）发病率和死亡率高，已成为全球最常见的肝癌。长链非编码 RNA 因其在人类疾病中发挥重要功能而被广泛研究。本研究旨在探讨在 HCC 中的作用和分子调控机制。实时定量聚合酶链反应检测 HCC 细胞中 、 和线粒体核糖体蛋白 S18a()的表达。细胞计数试剂盒-8（CCK-8）、集落形成、流式细胞术、JC-1、Transwell 和 Western blot 分析揭示了 在 HCC 中的功能。RNA 免疫沉淀（RIP）、RNA 下拉和荧光素酶报告基因分析验证了 、 和 之间的关联。核质分离实验揭示了 在 HCC 细胞中的亚细胞定位。根据 GEPIA 数据库显示， 在 HCC 组织样本中上调。在 HCC 细胞系中得到了一致的结果。随后，功能丧失实验证实 缺失抑制了细胞增殖、迁移、侵袭和上皮间质转化过程，并抑制了肿瘤生长。此外，作者验证了 在 HCC 中与 结合。 负调控 的表达。随后， 被鉴定为 的下游靶基因。最终，挽救实验表明 上调可以中和 缺陷引起的减弱效应。总之， 通过增加 的表达来海绵吸附 促进 HCC 进展。这些发现强调 作为 HCC 的一种新的治疗靶点。
