Condorelli Rosita A, Barbagallo Federica, Calogero Aldo E, Cannarella Rossella, Crafa Andrea, La Vignera Sandro
Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy.
J Clin Med. 2020 May 7;9(5):1373. doi: 10.3390/jcm9051373.
The use of inositols in endocrinological clinical practice is increasingly widespread. Most of the existing evidence concerns myoinositol (MYO), the most abundant form in nature, especially in women with polycystic ovarian syndrome. We have previously shown that MYO increases sperm motility in patients with asthenozoospermia by the increase of sperm mitochondrial membrane potential (MMP), a biofunctional sperm parameter closely associated to sperm motility. The aim of this study was to evaluate the effects of D-chiro-inositol (DCI), another biologically active isoform of inositols, on sperm MMP, as data on this matter has never been released so far. To accomplish this, semen samples from 15 patients with asthenozoospermia and 15 healthy normozoospermic men were incubated with increasing concentrations of DCI (0, 75, and 750 µg/mL) or phosphate buffer saline for 30 min. Incubation with DCI significantly improved sperm MMP at lower concentrations, and with shorter incubation length than those used in our similar MYO studies. In conclusion, these findings indicate that DCI positively impacts on sperm mitochondrial function in vitro. Studies aimed at assessing the role of DCI in the treatment of asthenozoospermia in-vivo are warranted.
肌醇在内分泌临床实践中的应用越来越广泛。现有的大多数证据都与肌醇（MYO）有关，它是自然界中最丰富的形式，尤其是在多囊卵巢综合征女性中。我们之前已经表明，MYO通过增加精子线粒体膜电位（MMP）来提高弱精子症患者的精子活力，MMP是一个与精子活力密切相关的生物功能精子参数。本研究的目的是评估肌醇的另一种生物活性异构体D-手性肌醇（DCI）对精子MMP的影响，因为迄今为止关于这方面的数据从未公布过。为了实现这一目标，将15例弱精子症患者和15例健康正常精子男性的精液样本与浓度递增的DCI（0、75和750μg/mL）或磷酸盐缓冲盐水孵育30分钟。与DCI孵育在较低浓度下显著改善了精子MMP，且孵育时间比我们类似的MYO研究中使用的时间更短。总之，这些发现表明DCI在体外对精子线粒体功能有积极影响。有必要开展旨在评估DCI在体内治疗弱精子症作用的研究。
