Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.
Department of Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Emerg Microbes Infect. 2020 Dec;9(1):1011-1022. doi: 10.1080/22221751.2020.1763209.
carbapenemase (KPC)-producing (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and -IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of -IncF plasmids are favored by CG258 it was of interest to examine the co-distribution of CRISPR and -IncF plasmids in such isolates. We collected 459 clinical in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and -positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in could effectively hindered -IncF plasmids invasion and existence. Notably, most -IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of in populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of -IncF plasmids in CG258 .
产碳青霉烯酶 (KPC)-的 (KPC-KP) 已在全球传播，并成为公共卫生的主要威胁。具有流行病学意义的是，KPC-KP 的国际大流行主要与 CG258 分离株和 -IncF 质粒有关。CRISPR-Cas 系统是一种适应性免疫系统，可以阻止水平基因转移驱动的基因扩展。由于 -IncF 质粒受到 CG258 的青睐，因此研究此类分离株中 CRISPR 和 -IncF 质粒的共同分布情况很有意思。我们收集了中国的 459 株临床分离株，并从 GenBank 中收集了 203 株全球全基因组序列，以确定 CRISPR-Cas 系统的流行情况。我们观察到 CRISPR-Cas 系统在 CG258 谱系和 -阳性分离株中明显稀缺。此外，接合和质粒稳定性测定的结果充分证明了 中的 CRISPR-Cas 系统可以有效阻止 -IncF 质粒的入侵和存在。值得注意的是，由于携带匹配和功能的原间隔区和 PAM，大多数 -IncF 质粒也被证明是 CRISPR 的良好靶标。总的来说，我们的工作表明，I-E 型 CRISPR-Cas 系统可能会影响 中 传播，而 CRISPR-Cas 系统的稀缺性是导致 CG258 中 -IncF 质粒传播的潜在因素之一。
