进化上古老的 BAH-PHD 蛋白介导 Polycomb 沉默。
Evolutionarily ancient BAH-PHD protein mediates Polycomb silencing.
机构信息
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403.
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894.
出版信息
Proc Natl Acad Sci U S A. 2020 May 26;117(21):11614-11623. doi: 10.1073/pnas.1918776117. Epub 2020 May 11.
Abstract
Methylation of histone H3 lysine 27 (H3K27) is widely recognized as a transcriptionally repressive chromatin modification but the mechanism of repression remains unclear. We devised and implemented a forward genetic scheme to identify factors required for H3K27 methylation-mediated silencing in the filamentous fungus and identified a bromo-adjacent homology (BAH)-plant homeodomain (PHD)-containing protein, EPR-1 (effector of polycomb repression 1; NCU07505). EPR-1 associates with H3K27-methylated chromatin, and loss of EPR-1 de-represses H3K27-methylated genes without loss of H3K27 methylation. EPR-1 is not fungal-specific; orthologs of EPR-1 are present in a diverse array of eukaryotic lineages, suggesting an ancestral EPR-1 was a component of a primitive Polycomb repression pathway.
摘要
组蛋白 H3 赖氨酸 27（H3K27）的甲基化被广泛认为是一种转录抑制性染色质修饰，但抑制机制尚不清楚。我们设计并实施了一项正向遗传学方案，以鉴定丝状真菌中 H3K27 甲基化介导沉默所需的因子，并鉴定出一种溴相邻同源（BAH）-植物同源域（PHD）包含蛋白，EPR-1（多梳抑制效应因子 1；NCU07505）。EPR-1 与 H3K27 甲基化染色质相关联，并且 EPR-1 的缺失会解除 H3K27 甲基化基因的抑制，而不会失去 H3K27 甲基化。EPR-1 不是真菌特异性的；EPR-1 的同源物存在于各种真核生物谱系中，表明原始 EPR-1 是原始多梳抑制途径的一个组成部分。
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