Egelkrout Erin, Hayden Celine, Fake Gina, Keener Todd, Arruda Paulo, Saltzman Ryan, Walker John, Howard John
Applied Biotechnology Institute, San Luis Obispo, CA USA.
VRI, Ames, IA USA.
Plant Cell Tissue Organ Cult. 2020;142(1):79-86. doi: 10.1007/s11240-020-01835-0. Epub 2020 May 11.
Porcine Epidemic Diarrhea Virus (PEDV) causes severe diarrhea and mortality in piglets. Robust immunity may break the transmission cycle. Expression of antigens in maize grains is a promising method for producing low-cost vaccines. As a first step, we expressed maize constructs containing PEDV S1 spike protein targeted to various cellular locations including the cell wall, endoplasmic reticulum, and vacuole, and fused to carrier proteins heat labile subunit (LTB) and a dendritic cell (DC) binding peptide, and obtained sufficient antigen for oral immunization. Constructs targeting S1 to the ER or fused to carrier proteins produced high levels of antigen of greater than 20 mg/kg. Oral administration to pigs elicited serum neutralizing antibodies, supporting oral immunization as a practical and cost-effective PEDV vaccine.
猪流行性腹泻病毒（PEDV）可导致仔猪严重腹泻和死亡。强大的免疫力可能会打破传播循环。在玉米籽粒中表达抗原是生产低成本疫苗的一种有前景的方法。作为第一步，我们构建了含有靶向各种细胞位置（包括细胞壁、内质网和液泡）的PEDV S1刺突蛋白的玉米表达载体，并与载体蛋白不耐热肠毒素亚基（LTB）和树突状细胞（DC）结合肽融合，获得了足够用于口服免疫的抗原。将S1靶向内质网或与载体蛋白融合的构建体产生了高于20mg/kg的高水平抗原。对猪进行口服给药可诱导血清中和抗体，支持口服免疫作为一种实用且具有成本效益的PEDV疫苗。
