Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Institute of Tropical Diseases, Universitas Airlangga, Surabaya, Indonesia.
Helicobacter. 2020 Aug;25(4):e12695. doi: 10.1111/hel.12695. Epub 2020 May 12.
The profile of gastric mucosal microbiota has not yet been described in the Indonesian population where the prevalence of Helicobacter pylori is low.
This is a cross-sectional study analyzing 16S rRNA of 137 gastric biopsy specimens. We analyzed the association between gastric microbiota, H. pylori infection, and gastric mucosal damage.
Among 137 analyzed samples, 27 were H. pylori-positive and 110 were H. pylori -negative based on culture, histology, and 16S rRNA gene analysis. Significantly lower α-diversity parameters, including Pielou's index, was observed in H. pylori-infected individuals compared with noninfected individuals (all P < .001). Among H. pylori-negative individuals, the permutational analysis of variance of Bray-Curtis dissimilarity distances showed a significant association with different ethnicities, suggesting some ethnic groups had specific microbiota profiles based on the presence of different operational taxonomic units. The linear discriminant analysis effect size (LEfSe) of the H. pylori-negative group showed significant associations between the presence of Micrococcus luteus and Sphingomonas yabuuchiae with Timor and Papuan ethnicities, respectively. The presence of Bulledia sp and Atopobium sp was associated with the Javanese ethnicity. We observed lower α-diversity scores in individuals with gastric mucosal damage and profiles with high abundances of Paludibacter sp and Dialister sp based on LEfSe analysis.
Our findings suggest the presence of H. pylori is more correlated with a distinct microbiome profile than ethnic precedence.
在印度尼西亚，幽门螺杆菌的流行率较低，尚未描述胃黏膜微生物群的特征。
这是一项横断面研究，分析了 137 个胃活检标本的 16S rRNA。我们分析了胃微生物群、幽门螺杆菌感染和胃黏膜损伤之间的关系。
在分析的 137 个样本中，根据培养、组织学和 16S rRNA 基因分析，27 个为幽门螺杆菌阳性，110 个为幽门螺杆菌阴性。与未感染个体相比，感染个体的α多样性参数（包括皮氏指数）显著降低（均 P<.001）。在幽门螺杆菌阴性个体中，Bray-Curtis 不相似距离的置换方差分析显示与不同种族存在显著关联，提示某些种族存在基于不同操作分类单位的特定微生物群特征。幽门螺杆菌阴性组的线性判别分析效应量（LEfSe）显示，黄色微球菌和黄杆菌分别与帝汶族和巴布亚族存在显著关联。布氏杆菌和咽峡炎菌的存在与爪哇族有关。我们观察到，胃黏膜损伤患者的α多样性评分较低，根据 LEfSe 分析，胃黏膜损伤患者的微生物群中存在大量 Paludibacter sp 和 Dialister sp。
我们的研究结果表明，幽门螺杆菌的存在与独特的微生物群特征比种族更相关。
