高效液相色谱-串联质谱法检测坚果中 spp. 产生的选定毒性代谢物。
HPLC-MS/MS Method for the Detection of Selected Toxic Metabolites Produced by spp. in Nuts.
机构信息
Centre of Competence for the Innovation in the agro-environmental Sector (AGROINNOVA), University of Torino, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy.
Department of Agricultural, Forestry and Food Sciences (DISAFA), University of Torino, Largo P. Braccini 2, 10095 Grugliasco (TO), Italy.
出版信息
Toxins (Basel). 2020 May 8;12(5):307. doi: 10.3390/toxins12050307.
spp. are emerging as producers of mycotoxins and other toxic metabolites in nuts. A HPLC-MS/MS method was developed to detect 19 metabolites produced by spp. on chestnuts, hazelnuts, walnuts and almonds. Two extraction methods were developed, one for chestnuts and one for the other three nuts. The recovery, LOD, LOQ and matrix effect were determined for each analyte and matrix. Correlation coefficients were always >99.99%. In walnuts, a strong signal suppression was observed for most analytes and patulin could not be detected. Six strains: , , , , and , isolated from chestnuts, were inoculated on four nuts. Chestnuts favored the production of the largest number of toxic metabolites. The method was used for the analysis of 41 commercial samples: 71% showed to be contaminated by -toxins. Cyclopenin and cyclopenol were the most frequently detected metabolites, with an incidence of 32% and 68%, respectively. Due to the risk of contamination of nuts with toxins, future studies and legislation should consider a larger number of mycotoxins.
spp. 正在成为坚果中霉菌毒素和其他有毒代谢物的生产者。开发了一种 HPLC-MS/MS 方法来检测 spp. 在栗子、榛子、核桃和杏仁上产生的 19 种代谢物。开发了两种提取方法，一种用于栗子，另一种用于其他三种坚果。确定了每个分析物和基质的回收率、LOD、LOQ 和基质效应。相关系数始终>99.99%。在核桃中，大多数分析物观察到强烈的信号抑制，并且无法检测到棒曲霉素。从栗子中分离出的六种菌株：,,,, 和 ，接种在四种坚果上。栗子有利于产生最多数量的 有毒代谢物。该方法用于分析 41 个商业样本：71%的样本显示被 - 毒素污染。环匹罗菌素和环匹罗醇是最常检测到的代谢物，分别为 32%和 68%。由于坚果受到毒素污染的风险，未来的研究和法规应考虑更多种类的霉菌毒素。
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