超高效液相色谱-串联质谱法快速测定食用植物油中的真菌毒素。
A rapid method for the determination of mycotoxins in edible vegetable oils by ultra-high performance liquid chromatography-tandem mass spectrometry.
机构信息
Department of Chemistry and Physics, Analytical Chemistry Area, University of Almería, Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence ceiA3, Carretera de Sacramento s/n, E-04120 Almería, Spain.
Department of Chemistry and Physics, Analytical Chemistry Area, University of Almería, Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence ceiA3, Carretera de Sacramento s/n, E-04120 Almería, Spain.
出版信息
Food Chem. 2019 Aug 1;288:22-28. doi: 10.1016/j.foodchem.2019.03.003. Epub 2019 Mar 4.
An analytical method based on a QuEChERS procedure (quick, easy, cheap, effective, rugged and safe) has been developed for the determination of mycotoxins (α-zearalenol and zearalenone, and aflatoxins B1, B2, G1 and G2) in edible oils. The analysis was performed by ultra-high performance liquid chromatography coupled to triple quadrupole analyser (UHPLC-QqQ-MS/MS). The method was fully validated and the quantification limit is 0.5 μg kg for aflatoxins and 1 μg kg for α-zearalenol and zearalenone. Suitable recoveries were obtained at low concentration levels (0.5-25 μg kg for aflatoxins and 1-25 μg kg for α-zearalenol and zearalenone), ranging from 80 to 120%. Intra and inter-day precision values were also evaluated and relative standard deviation was lower than 20%. The expanded uncertainty, U, was also evaluated ant it was below 32% at 25 µg kg. The validated method has been applied to monitor the presence of mycotoxins in 194 samples belonging to different types of edible oils (olive oil, sunflower oil, soy oil and corn oil). Zearalenone was detected in 25% of the analysed samples at concentrations up to 25.6 μg kg, and aflatoxin G1 and G2 in 3% and 14% of the samples at a maximum concentration of 1.9 and 6.8 μg kg respectively.
已开发出一种基于 QuEChERS 程序（快速、简便、廉价、有效、耐用和安全）的分析方法，用于测定食用油中的霉菌毒素（α-玉米赤霉醇和玉米赤霉烯酮，以及黄曲霉毒素 B1、B2、G1 和 G2）。分析采用超高效液相色谱-三重四极杆串联质谱法（UHPLC-QqQ-MS/MS）进行。该方法经过全面验证，黄曲霉毒素的定量限为 0.5μg/kg，α-玉米赤霉醇和玉米赤霉烯酮的定量限为 1μg/kg。在低浓度水平（黄曲霉毒素为 0.5-25μg/kg，α-玉米赤霉醇和玉米赤霉烯酮为 1-25μg/kg）下，回收率适宜，范围在 80-120%之间。还评估了日内和日间精密度，相对标准偏差低于 20%。还评估了扩展不确定度，U，在 25μg/kg 时低于 32%。经验证的方法已应用于监测 194 份属于不同类型食用油（橄榄油、葵花籽油、大豆油和玉米油）的霉菌毒素的存在情况。在分析的样品中，有 25%的样品检测到玉米赤霉烯酮，浓度高达 25.6μg/kg，有 3%和 14%的样品检测到黄曲霉毒素 G1 和 G2，最大浓度分别为 1.9μg/kg 和 6.8μg/kg。
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