Ren Hui, Li Xianbi, Li Yujie, Li Mengjun, Sun Jiyuan, Wang Fanlong, Zeng Jianyan, Chen Yang, Wang Lei, Yan Xingying, Fan Yanhua, Jin Dan, Pei Yan
Biotechnology Research Center, Southwest University, Chongqing, China.
Front Plant Sci. 2022 Aug 22;13:944364. doi: 10.3389/fpls.2022.944364. eCollection 2022.
Four P4-ATPase flippase genes, , and were identified in , one of the most devastating phytopathogenic fungi in the world. Knock out of , and , or knock down of significantly decreased the pathogenicity of the mutants in cotton. Among the mutants, the greatest decrease in pathogenicity was observed in Δ. VdDrs2 was localized to plasma membrane, vacuoles, and -Golgi network (TGN). In vivo observation showed that the infection of the cotton by Δ was significantly delayed. The amount of two known Verticillium toxins, sulfacetamide, and fumonisin B1 in the fermentation broth produced by the Δ strain was significantly reduced, and the toxicity of the crude Verticillium wilt toxins to cotton cells was attenuated. In addition, the defect of VdDrs2 impaired the synthesis of melanin and the formation of microsclerotia, and decreased the sporulation of . Our data indicate a key role of P4 ATPases-associated vesicle transport in toxin secretion of disease fungi and support the importance of mycotoxins in the pathogenicity of .
在世界上最具破坏性的植物病原真菌之一的大丽轮枝菌中，鉴定出了四个P4-ATPase翻转酶基因，即 、 、 和 。敲除 、 和 ，或敲低 ，均显著降低了突变体在棉花中的致病性。在这些突变体中，Δ 的致病性下降最为显著。VdDrs2定位于质膜、液泡和反式高尔基体网络（TGN）。体内观察表明，Δ 对棉花的感染显著延迟。Δ 菌株产生的发酵液中两种已知的大丽轮枝菌毒素，即磺酰胺和伏马菌素B1的含量显著降低，且大丽轮枝菌枯萎病粗毒素对棉花细胞的毒性减弱。此外，VdDrs2的缺陷损害了黑色素的合成和微菌核的形成，并降低了大丽轮枝菌的产孢量。我们的数据表明，P4 ATP酶相关的囊泡运输在致病真菌的毒素分泌中起关键作用，并支持霉菌毒素在大丽轮枝菌致病性中的重要性。
