Section of Respiratory Disease, Department of Clinical Medicine and Surgery, Monaldi Hospital, Federico II University, 80138 Naples, Italy.
Staff of The United Nations Educational, Scientific and Cultural Organization (UNESCO) Chair on Health Education and Sustainable Development, Federico II University, 80131, Naples, Italy.
Int J Environ Res Public Health. 2020 May 8;17(9):3289. doi: 10.3390/ijerph17093289.
Governments and clinicians that were fully involved in the dramatic SARS-CoV-2 outbreak during the last few weeks in Italy (and more or less all over the world) are fiercely debating the use of methods for screening this viral infection. Thus, all countries are employing a lot of resources in order to test more and more subjects. For this purpose, there are different strategies, based on either direct or indirect tests. Among the first category, the main assays used for SARS-CoV-2 are based on a real-time reverse transcriptase polymerase chain reaction (RT-PCR). Such tests can be performed on nasopharyngeal and oropharyngeal swabs for the categories of those with symptoms and those potentially exposed. In order to integrate the molecular assays in the diagnosis of SARS-CoV-2, a wide range of serology immunoassays (IAs) have also been developed. If we want to identify "immune" people in order to let them to come back to work, serology is the best (and probably the only) approach.
在过去几周中，意大利（以及全球各地）的政府和临床医生全面参与了 SARS-CoV-2 疫情的防控，他们正在激烈讨论筛查这种病毒感染的方法。因此，各国都投入了大量资源，以便对越来越多的人群进行检测。为此，有不同的策略，基于直接或间接检测。在第一类中，用于 SARS-CoV-2 的主要检测方法基于实时逆转录聚合酶链反应（RT-PCR）。这些检测可以对有症状和潜在暴露的人群进行鼻咽和口咽拭子检测。为了将分子检测纳入 SARS-CoV-2 的诊断中，还开发了广泛的血清学免疫测定（IA）。如果我们想识别出“免疫”人群，以便让他们重返工作岗位，那么血清学是最佳（可能也是唯一）的方法。
