Faculty of Life and Earth Sciences, Jagannath University, Dhaka 1100, Bangladesh.
Faculty of Biology, Technische Universität Dresden, D-01062 Dresden, Germany.
Genes (Basel). 2020 May 9;11(5):527. doi: 10.3390/genes11050527.
Bioinformatic and molecular characterization of satellite repeats was performed to understand the impact of their diversification on genome evolution. Satellite repeat diversity was evaluated in four cultivated and wild species, including the diploid species and , as well as the tetraploid species and . We comparatively characterized six satellite repeat families using in total 76 clones with 180 monomers. We observed that the monomer units of VaccSat1, VaccSat2, VaccSat5, and VaccSat6 showed a higher order repeat (HOR) structure, likely originating from the organization of two adjacent subunits with differing similarity, length and size. Moreover, VaccSat1, VaccSat3, VaccSat6, and VaccSat7 were found to have sequence similarity to parts of transposable elements. We detected satellite-typical tandem organization for VaccSat1 and VaccSat2 in long arrays, while VaccSat5 and VaccSat6 distributed in multiple sites over all chromosomes of tetraploid , presumably in long arrays. In contrast, very short arrays of VaccSat3 and VaccSat7 are dispersedly distributed over all chromosomes in the same species, likely as internal parts of transposable elements. We provide a comprehensive overview on satellite species specificity in , which are potentially useful as molecular markers to address the taxonomic complexity of the genus, and provide information for genome studies of this genus.
进行了卫星重复序列的生物信息学和分子特征分析，以了解它们多样化对基因组进化的影响。在四个栽培和野生物种中评估了卫星重复序列的多样性，包括二倍体物种 和 ，以及四倍体物种 和 。我们使用总共 76 个克隆和 180 个单体比较了六个卫星重复家族的特征。我们观察到，VaccSat1、VaccSat2、VaccSat5 和 VaccSat6 的单体单元表现出更高阶重复（HOR）结构，可能源自两个具有不同相似性、长度和大小的相邻亚基的组织。此外，VaccSat1、VaccSat3、VaccSat6 和 VaccSat7 被发现与转座元件的部分序列具有相似性。我们在长数组中检测到 VaccSat1 和 VaccSat2 的卫星典型串联组织，而 VaccSat5 和 VaccSat6 分布在四倍体 所有染色体的多个位点上，可能以长数组的形式存在。相比之下，VaccSat3 和 VaccSat7 的非常短的数组则分散分布在同一物种的所有染色体上，可能是转座元件的内部部分。我们提供了 中卫星种特异性的综合概述，这些卫星可能作为分子标记用于解决该属的分类复杂性，并为该属的基因组研究提供信息。
