Inchingolo Francesco, Martelli Francesco Saverio, Gargiulo Isacco Ciro, Borsani Elisa, Cantore Stefania, Corcioli Fabiana, Boddi Anna, Nguyễn Kieu C D, De Vito Danila, Aityan Sergey K, Pham Van Hung, Dipalma Gianna, Ballini Andrea
Department Interdisciplinary of Medicine, University of Bari "Aldo Moro", 70121 Bari, Italy.
International Microdentistry Institute (IMI), 50139 Florence, Italy.
Biomedicines. 2020 May 9;8(5):115. doi: 10.3390/biomedicines8050115.
Chronic periodontitis (CP) is a complex pathology with a significant impact worldwide causing bone loss. Oral dysbiosis is a highly inflammatory condition associated to a long-term insulting infection and represents an underestimated CP key factor associated with an imbalance of pro-inflammatory and anti-inflammatory gene responses. The presence of a single nucleotide polymorphisms (SNPs) in the promoter region of interleukin 10 (IL-10) gene-1082, -819, and -592 was a possible determinant cause. This translational research aimed to provide outcomes on the role of IL-10 gene expression in bone loss diseases in patients affected by CP. Caucasian patients ( = 96) affected by CP were recruited from the Italian population. The subgingival samples were collected using the Bacterial Periodontal Assessment by Biomolecular Diagnostic and the characterization of a set of 15 bacterial DNA responsible of periodontitis was performed by real-time multiplex PCR. In addition, two viruses, Epstein-Barr Virus (EBV) and Herpes Simplex Virus 1 (HSV-1), and a pathogenic fungi () were included as a part of our panel. Our results confirmed an existing association between IL-10 gene polymorphisms and polymorphism of tumor necrosis factor alpha (TNFα), interleukin 1α-β-RN (IL-1α-β-RN), collagen type-l alpha (COLIA1), and vitamin D receptor (VDRs) genes in CP. Further studies are needed to improve diagnosis and endorse more effective therapeutic procedures for periodontal disease.
慢性牙周炎（CP）是一种复杂的病理状况，在全球范围内造成骨丢失，影响重大。口腔生态失调是一种与长期感染相关的高度炎症状态，是与促炎和抗炎基因反应失衡相关的、被低估的慢性牙周炎关键因素。白细胞介素10（IL-10）基因启动子区域-1082、-819和-592处单核苷酸多态性（SNP）的存在可能是决定性原因。这项转化研究旨在提供关于IL-10基因表达在慢性牙周炎患者骨丢失疾病中作用的结果。从意大利人群中招募了96名患慢性牙周炎的白种人患者。使用生物分子诊断法进行细菌牙周评估收集龈下样本，并通过实时多重PCR对一组导致牙周炎的15种细菌DNA进行特征分析。此外，我们的检测组还包括两种病毒，即爱泼斯坦-巴尔病毒（EBV）和单纯疱疹病毒1型（HSV-1），以及一种致病真菌。我们的结果证实了慢性牙周炎中IL-10基因多态性与肿瘤坏死因子α（TNFα）、白细胞介素1α-β-RN（IL-1α-β-RN）、I型胶原α（COLIA1）和维生素D受体（VDRs）基因多态性之间存在关联。需要进一步研究以改善牙周疾病的诊断并支持更有效的治疗方法。
